Anti-EHMT1 Antibody

EHMT1 (euchromatic histone methyltransferase 1), also known as G9a-like protein (GLP1), is a histone methyltransferase. This protein specifically methylates Lysine 9 of histone H3 in vitro. H3Lys9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is thought to be targeted to histone H3 by different DNA-binding proteins including E2F6, MGA, MAX and/or DP1. During G0 phase, it contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle. EMHT1/GLP1 is phosphorylated upon DNA damage, probably by ATM or ATR. Defects in EHMT1 result in severe mental retardation, hypotonia, and epileptic seizures.

Not tested in other species.

Epitope: Internal

The immunogen was a KLH-conjugated synthetic peptide corresponding to an internal region of human EHMT1.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Chromatin Biology

Use Anti-EHMT1 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect EHMT1 also known as H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5.

Western Blot Analysis
This antibody recognized EMHT1 in transfected 293 cell lysates.


ELISA:
This antibody is reported by an
outside laboratory to be suitable for ELISA

Immunocytochemistry
Detects EHMT1 in the nuclei of A431, HeLa, or NIH/3T3 cells in immunofluorescent analysis.

~138 kDa

Protein A chromatography

Format: Purified

Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
A431, HeLa, or NIH/3T3 cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.