Anti-FOXO3A Antibody

Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia.

This antibody recognizes FOXO3A.

Synthetic peptide corresponding to FOX03A

Immunocytochemistry:
A 1:500 dilution of this antibody was used to detect FOXO3A in HeLa cells. Positive staining in the nucleus.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Transcription Factors

This Anti-FOXO3A Antibody is validated for use in WB, IC for the detection of FOXO3A.

Western Blot Analysis:
1:500 dilution of this antibody was used to detect FOXO3A in Jurkat cell lysate.

~80 kDa

Replaces: 04-1007

Affinity purified

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Control
FOXO3A in Jurkat cell lysate.

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