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Merck
CN

07-164

抗磷酸化H2A.X(Ser139)抗体

Upstate®, from rabbit

别名:

H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

抗磷酸化H2A.X(Ser139)抗体, Upstate®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, vertebrates

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

phosphorylation (pSer139)

Quality Level

Gene Information

human ... HIST2H2AC(8338)

Analysis Note

对照
星形孢菌素处理的Jurkat裂解液、紫外线处理的HT-29细胞、阿霉素处理的HT-29细胞。
已通过蛋白质印迹对未经处理和经星形孢菌素处理的Jurkat裂解液进行了常规评估。

蛋白质印迹分析:
该批次产品以1:500的稀释度在10 μg星形孢菌素处理的Jurkat裂解液中检测到磷酸化H2A.X。

Application

免疫沉淀
先前批次的该抗体已由独立实验室成功地使磷酸化的H2A.X免疫沉淀。
抗磷酸化H2A.X(Ser139)抗体是一种兔多克隆抗体,可用于检测磷酸化H2A.X(Ser139)(也被称为组蛋白2 H2ac、组蛋白IIa) & 已在IP&和WB中进行了验证。

Biochem/physiol Actions

在Ser139处磷酸化的组蛋白H2A.X。
基于同源序列的保守型,预测其会发生广泛的物种交叉反应。

General description

组蛋白H2A是参与真核细胞染色质结构的5种主要组蛋白之一。H2A具有一个主要的球状结构域和一个长N末端的尾巴,其与核糖体核小体′串珠′结构体的结构有关。
15kda

Immunogen

表位:aa134-142

Other Notes

替代品:MABE205
浓度:请参考批次特异性浓缩物的分析证书。

Physical form

形式:纯化

Preparation Note

自收到之日起,在 -20°C 条件下可稳定保存 1 年。
处理建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。
避免反复冻融,其可能会破坏IgG并影响产品性能。注意:冷藏室温度变化至低于-20°C时可能导致-含甘油的溶液在储存过程中冻结。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1

法规信息

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