Anti-SIRT3 (CT) Antibody

SIRT3 is a member of the class I sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT3 is located in the mitochondria and has been implicated in regulating metabolic processes. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of Sirt3 in cultured cells increases respiration and decreases the production of reactive oxygen species. Fasting increases Sirt3 expression in white and brown adipose tissue. A longer form of SIRT3 is present in the nucleus where it exhibits histone deacetylase activity. SIRT3 protects cardiomyocytes from stress mediated cell death due to deacetylation of a nuclear factor, Ku-70.

Not tested in other species.

This antibody recognizes SIRT3. Endogenous levels of this protein vary among cell lines; the protein was easily detected in RAW264.7 and C2C12 cells but only weakly detected in HeLa cells, and not detected in Jurkat, HEK293 or PC3 cells.

Epitope: C-terminus

The immunogen was a peptide corresponding the C-terminal region of human SIRT3.

Anti-SIRT3 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of SIRT3 (C-terminus) also known sirtuin 3 & has been validated in WB, ELISA & IHC.

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Western Blot Analysis:
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.

44 kDa

Purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.09% NaN3.

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
C2C12 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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