推荐产品
生物来源
rabbit
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human, mouse
技术
dot blot: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
基因信息
human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)
一般描述
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The phosphorylated form of H2A.X is thought to be invloved with DNA repair, possibly through reorganizing chromatin into a more open state and allowing the recruitment of proteins to repair DNA double stranded breaks.
免疫原
KLH-conjugated linear peptide corresponding to the C-terminus of human phospho Histone H2A.X phosphorylated at Tyr142.
应用
Anti-phospho Histone H2A.X Antibody (Tyr142) is a highly specific rabbit polyclonal antibody, that targets Histone H2A.X & has been tested in western blotting & Dot Blot.
Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H2A.X (Tyr142) peptides, but not phospho Histone H2A.X (Tyr142) peptides or other peptides corresponding to modified histones.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
质量
Evaluated by Western Blotting in colcemid treated HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2A.X (Tyr142) in colcemid treated HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2A.X (Tyr142) in colcemid treated HeLa cell lysate.
目标描述
~16 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
外形
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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