Anti-phospho-IRS-2 (Ser388) Antibody

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

This antibody recognizes IRS-2 phosphorylated at Ser388.

Epitope: Phosphorylated Ser388

KLH-conjugated linear peptide corresponding to human IRS-2 phosphorylated Ser388.

Anti-phospho-IRS-2 (Ser388) Antibody detects level of phospho-IRS-2 (Ser388) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.

Peptide Inhibition Assay Analysis: 10 µg from a representative lot blocked IRS-2 on insulin treated HEK293 cells.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Evaluated by Western Blot in untreated and insulin treated HEK293 cell lysates.

Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.

~160 kDa observed. The calculated molecular weight is 137 kDa, however IRS-2 has been shown as a ~160 kDa band in western blots (Rother, K., et al. (1998). Journal of Biological Chemistry. 273(28):17491–17497.)

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Untreated and insulin treated HEK293 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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