07-1507
Anti-phospho-Mypt1 (Ser507) Antibody
from rabbit, purified by affinity chromatography
别名:
Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit
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所有图片(1)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推荐产品
生物来源
rabbit
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse
种属反应性（根据同源性预测）
human (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)
技术
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pSer507)
基因信息
human ... PPP1R12A(4659)
一般描述
Myosin phosphatase targeting subunit 1 (MYPT1) is a regulatory targeting subunit that is thought to be responsible for targeting the holoenzyme to the proper positions in muscle and moving PP1 to myosin. MYPT1 is the major subunit involved in myosin light chain phosphatase (MLCP). It is also essential for vasodilation caused by nitric oxide (NO). A decrease in MYPT1 has been shown to play a role in increased tolerance of NO in pulmonary arteries.
特异性
Other homologies: Rat (89% sequence homology).
This antibody recognizes Mypt1 phosphorylated at (Ser507).
免疫原
Epitope: Phosphorylated (Ser507)
KLH-conjugated linear peptide corresponding to human Mypt1 phosphorylated at (Ser507).
应用
Research Category
Signaling
Cell Structure
Signaling
Cell Structure
Research Sub Category
Developmental Signaling
Muscle Physiology
Developmental Signaling
Muscle Physiology
This Anti-phospho-Mypt1 (Ser507) Antibody is validated for use in WB for the detection of phospho-Mypt1 (Ser507).
质量
Evaluated by Western Blot in untreated and PMA-treated NIH/3T3 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Mypt1 in 10 µg of untreated and PMA-treated NIH/3T3 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Mypt1 in 10 µg of untreated and PMA-treated NIH/3T3 cell lysates.
目标描述
~120 kDa observed.
Uniprot gives a calculated molecular weight of 115 kDa, which can be observed at higher molecular weights due to hyperphosphorylation. Uncharacterized bands appear at ~30 kDa and ~130 kDa in some lysates.
Uniprot gives a calculated molecular weight of 115 kDa, which can be observed at higher molecular weights due to hyperphosphorylation. Uncharacterized bands appear at ~30 kDa and ~130 kDa in some lysates.
外形
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Untreated and PMA-treated NIH/3T3 cell lysates
Untreated and PMA-treated NIH/3T3 cell lysates
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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