推荐产品
生物来源
rabbit
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, rat, mouse
技术
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... RAC1(5879)
一般描述
Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.
特异性
Recognizes Rac1, does not cross react with Cdc42. Rac1 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Rac regulates actin polymerization to form lamellipodia.
免疫原
Synthetic peptide corresponding to amino acids 130-141 of human Rac1 (KEKKLTPITYPQ-C).
应用
Anti-Rac1 Antibody is an antibody against Rac1 for use in WB.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
质量
Routinely evaluated by Western Blot on L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.
目标描述
21 kDa
联系
Replaces: AB4202
外形
Protein A purified
Format: Purified
Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative
储存及稳定性
Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Jeffrey M Hord et al.
Experimental gerontology, 83, 148-157 (2016-08-19)
Age-related loss of skeletal muscle mass and function, referred to as sarcopenia, is mitigated by lifelong calorie restriction as well as exercise. In aged skeletal muscle fibers there is compromised integrity of the cell membrane that may contribute to sarcopenia.
Joon-Il Jun et al.
Nature cell biology, 12(7), 676-685 (2010-06-08)
Cellular senescence is a recognized mechanism of tumour suppression; however, its contribution to other pathologies is not well understood. We show that the matricellular protein CCN1 (also known as CYR61; cysteine-rich protein 61), which is dynamically expressed at sites of
D Araiza-Olivera et al.
Oncogene, 37(7), 944-952 (2017-10-24)
Activating mutations in the RAC1 gene have recently been discovered as driver events in malignant melanoma. Expression of this gene is associated with melanocyte proliferation, and melanoma cells bearing this mutation are insensitive to BRAF inhibitors such as vemurafenib and
Jeffrey M Hord et al.
Physiological reports, 9(1), e14606-e14606 (2021-01-06)
Reduced mechanical loading results in atrophy of skeletal muscle fibers. Increased reactive oxygen species (ROS) are causal in sarcolemmal dislocation of nNOS and FoxO3a activation. The Nox2 isoform of NADPH oxidase and mitochondria release ROS during disuse in skeletal muscle.
Nicolas Bery et al.
Cell chemical biology, 26(11), 1544-1558 (2019-09-17)
The selective downregulation of activated intracellular proteins is a key challenge in cell biology. RHO small GTPases switch between a guanosine diphosphate (GDP)-bound and a guanosine triphosphate (GTP)-bound state that drives downstream signaling. At present, no tool is available to
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