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生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
pig, human
种属反应性(根据同源性预测)
porcine (based on 100% sequence homology), rabbit (based on 100% sequence homology), monkey (based on 100% sequence homology), opossum (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
技术
immunocytochemistry: suitable
western blot: suitable
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ECT2(1894)
一般描述
上皮细胞转化序列2癌基因(ECT2)是鸟嘌呤核苷酸交换因子或GEF,属于Rho GEF的Dbl家族。ECT2由N端内的2个BRCT域,一个DBL同源域和一个PH域组成。研究表明,ECT2定位于细胞皮层和中央纺锤体,并且它是细胞增生过程中卵裂沟形成的关键部分。已显示仅包含N末端结构域的裂解的ECT2的过表达会引起多核化。作为GEF,已显示它与Rac蛋白,RhoA和RhoC特异性结合。
特异性
该抗体可识别
C端的ECT2。
C端的ECT2。
免疫原
KLH缀合的线性肽,对应于人ECT2的C末端附近区域。
表位:C端
应用
抗ECT2抗体是针对ECT2 的抗体,可用于WB & IC。
研究子类别
G-蛋白
G-蛋白
研究类别
信号传导
信号传导
蛋白质印迹法(Snap i.d.)分析:5 µg/mL先前批次在A549细胞裂解液中检测到ECT2。
免疫细胞化学分析: 先前批次已被独立实验室用于Heya8细胞。(Der Channing,北卡罗来纳大学教堂山分校,NC,channing_der@med.unc.edu)
免疫细胞化学分析: 先前批次已被独立实验室用于Heya8细胞。(Der Channing,北卡罗来纳大学教堂山分校,NC,channing_der@med.unc.edu)
质量
已通过蛋白质印迹对A549细胞裂解液进行了评估。
蛋白质印迹分析:1 µg/mL该抗体可在10 µg的A549细胞裂解液中检测到ECT2。
蛋白质印迹分析:1 µg/mL该抗体可在10 µg的A549细胞裂解液中检测到ECT2。
目标描述
可观察到约105 kDa的条带
外形
亲和纯化
在含 0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl 和 0.05% 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。
储存及稳定性
自收到之日起,在2-8°C条件下可稳定保存1年。
分析说明
对照
A549细胞裂解液
A549细胞裂解液
其他说明
浓度:请参考批次特异性浓缩物的分析证书。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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