所有图片(3)
Neurogenic locus notch homolog protein 1 precursor, Notch (Drosophila) homolog 1 (translocation-associated), Notch 1, Notch homolog 1, translocation-associated (Drosophila), Translocation-associated notch protein TAN-1, Neurogenic locus notch homolog pro
推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human
请勿与下列物质发生反应
mouse
技术
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... NOTCH1(4851)
一般描述
Notch是单程跨膜受体蛋白家族,在内质网中以无活性形式合成,并在识别后被反式高尔基体网络中的弗林蛋白酶样转化酶(S1裂解)在细胞外部位点进行蛋白水解裂解。RQRR序列在以异二聚体的形式到达质膜之前产生活性的,配体可及的形式。Notch家族由4个成员(1-4)组成,其配体包括Delta和Jagged配体家族。这些配体引起Notch的蛋白水解以释放细胞内结构域。切割产生C末端片段N(TN)和N末端片段N(EC)。配体结合后,它被TNF-α转化酶(TACE)裂解(S2裂解),产生与膜相关的中间片段,称为Notch细胞外截短(NEXT)。然后,该片段被gly1743和val1744之间的早老素依赖性γ-分泌酶切割(S3切割),以从膜释放细胞内结构域(NICD)。该结构域易位至细胞核并通过结合DNA结合蛋白Csl启动转录事件。Notch家族成员参与细胞的分化和发育。
特异性
Notch 1,细胞内。对人Notch 2无反应性。
与其他物种的反应性尚未确定。
免疫原
来自人Notch 1的合成肽。
应用
ELISA:先前批次的1:20,000-1:100,000稀释液用于针对肽免疫原的标准夹心ELISA测定。
最佳工作稀释液必须由最终用户确定。
免疫组织化学(石蜡):
先前批次的代表性测试。
具有表位检索的最佳IGF-1染色:脂肪瘤。
最佳工作稀释液必须由最终用户确定。
免疫组织化学(石蜡):
先前批次的代表性测试。
具有表位检索的最佳IGF-1染色:脂肪瘤。
使用经ELISA、WB、IHC(P)验证的细胞内(兔多克隆抗体)抗Notch1抗体检测Notch1(也称为神经源性基因座notch同源蛋白1前体)。
质量
已通过蛋白质印迹对Hela裂解液进行了常规评估。
蛋白质印迹分析:该批次的产品已以1:500的稀释度在10 μg HeLa裂解液中检测到NOTCH1,细胞内(裂解形式)。
蛋白质印迹分析:该批次的产品已以1:500的稀释度在10 μg HeLa裂解液中检测到NOTCH1,细胞内(裂解形式)。
目标描述
~80 kDa
联系
替代:04-1046
外形
兔多克隆IgG血清溶于含有0.02 M磷酸钾、0.15 M NaCl和0.01%叠氮化钠的缓冲液中。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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