Anti-PP1γ1 Antibody

Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.

Recognizes Protein Phosphatase 1γ1. No cross reactivity with other recombinant PP1 isoforms.

Epitope: C-terminus

The immunogen used to the purified peptide conjugated to KLH corresponding to the to the C –terminus of PP1 gamma 1 catalytic subunit (amino acids 311-323).

Anti-PP1γ1 Antibody detects level of PP1γ1 & has been published & validated for use in WB & IP.

Immunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP-1 GAMMA on 10 μg of HeLa lysates.

~37 kDa

Replaces: AB4084

Purified

Format: Purified

Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa Cell Lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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