07-1217
Anti-PP1β Antibody
Upstate®, from rabbit
别名:
Serine/threonine-protein phosphatase PP1-beta catalytic subunit, Protein phosphatase 1, catalytic subunit, beta, Protein phosphatase 1, catalytic subunit, beta isoform, Protein phosphatase 1-beta, Protein phosphatase 1-delta
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所有图片(3)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源
rabbit
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
bovine, mouse, human, rat
制造商/商品名称
Upstate®
技术
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... PPP1CB(5500)
一般描述
Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
特异性
Recognizes Protein Phosphatase 1β. No cross reactivity with other recombinant PP1 isoforms.
免疫原
Epitope: C-terminus
The immunogen used to the purified peptide conjugated to KLH corresponding to the C-terminus of PP1 beta catalytic subunit (amino acids 318-327).
应用
Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-15μg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.
Immunohistochemistry(paraffin):
Optimal Staining of PP1beta Polyclonal Antibody: Human Testis PP1beta staining in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.
Research Category
Signaling
Signaling
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
This Anti-PP1β Antibody is validated for use in WB, IP, IH(P) for the detection of PP1β.
质量
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 μg of HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-1beta on 10 μg of HeLa lysates.
目标描述
~ 38kDa
联系
Replaces: AB4083
外形
Purified
Format: Purified
Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
HeLa Cell Lysate
HeLa Cell Lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Wen Li et al.
Journal of diabetes research, 2018, 8743874-8743874 (2018-06-30)
Ingested food is received, mixed, and ground into chyme by distinct gastric motility patterns. Diabetes impairs gastric muscle function, but the mechanisms underlying diabetes-induced gastric muscle dysfunction are unknown. Here, we compared the expression and phosphorylation of Ca2+ sensitization and
Ruijie Liu et al.
Journal of molecular and cellular cardiology, 87, 204-213 (2015-09-04)
There are 3 protein phosphatase 1 (PP1) catalytic isoforms (α, β and γ) encoded within the mammalian genome. These 3 gene products share ~90% amino acid homology within their catalytic domains but each has unique N- and C-termini that likely
Peng Xu et al.
The Journal of experimental medicine, 218(8) (2021-06-23)
Biochemical, pathogenic, and human genetic data confirm that GSAP (γ-secretase activating protein), a selective γ-secretase modulatory protein, plays important roles in Alzheimer's disease (AD) and Down's syndrome. However, the molecular mechanism(s) underlying GSAP-dependent pathogenesis remains largely elusive. Here, through unbiased
Ruijie Liu et al.
Acta biochimica et biophysica Sinica, 51(3), 323-330 (2019-02-06)
Protein phosphatase 1 isoforms α, β, and γ (PP1α, PP1β, and PP1γ) are highly homologous in the catalytic domains but have distinct subcellular localizations. In this study, we utilized both primary cell culture and knockout mice to investigate the isoform-specific
Pragya Sharma et al.
Diabetes, 65(9), 2606-2617 (2016-06-02)
Akt substrate of 160 kDa (AS160) phosphorylation on Thr(642) and Ser(588) by Akt is essential for insulin's full effect on glucose transport. However, protein phosphorylation is determined by the balance of actions by kinases and phosphatases, and the specific phosphatase(s)
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