06-942-AF555
Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor™ 555 Conjugate
from rabbit, ALEXA FLUOR™ 555
别名:
H3K9ac, Histone H3, Lys9-acetylated, H3 histone family, member T, Lys9-acetylated, Lys9-acetylated histone 3, histone cluster 3, Lys9-acetylated, Lys9-acetylated H3
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所有图片(5)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物来源
rabbit
质量水平
偶联物
ALEXA FLUOR™ 555
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
rat, mouse, human
技术
flow cytometry: suitable
immunocytochemistry: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
acetylation (Lys9)
基因信息
human ... HIST1H3F(8968)
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
免疫原
KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of histone H3 with acetylated Lys9.
应用
Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor™ 555 Conjugate, Cat. No. 06-942-AF555, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.
Flow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected nuclear histone H3 Lys9 acetylation (H3K9ac) in A431, HeLa, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected nuclear histone H3 Lys9 acetylation (H3K9ac) in A431, HeLa, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.
质量
Evaluated by Immunocytochemistry in HUVECs.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys9 acetylation (H3K9ac) in HUVECs.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys9 acetylation (H3K9ac) in HUVECs.
目标描述
~17 kDa observed.
外形
Purified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide.
其他说明
Concentration: Please refer to lot specific datasheet.
法律信息
ALEXA FLUOR is a trademark of Life Technologies
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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