生物来源
rabbit
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, mouse
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
mouse ... Clec1B(56760)
特异性
Recognizes IRAK.
免疫原
Peptide (DRQGPEESDEFQS) corresponding to amino acids 700-712 of human IRAK
应用
Detect IRAK using this Anti-IRAK Antibody validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
质量
routinely evaluated by immunoblot on HeLa nuclear extract
目标描述
80 kDa
外形
Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
储存及稳定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
Jean Hu et al.
Journal of immunology (Baltimore, Md. : 1950), 168(8), 3910-3914 (2002-04-09)
IL-1R-associated kinase (IRAK) plays a pivotal role in IL-1R/Toll-like receptor (TLR)-mediated signaling and NF-kappaB activation. IRAK from leukocytes undergoes rapid activation and inactivation/degradation following IL-1 or LPS stimulation. The rapid degradation of IRAK may serve as a negative feedback mechanism
Cigarette smoke induces CXCL8 production by human neutrophils via activation of TLR9 receptor.
Mortaz, E; Adcock, IM; Ito, K; Kraneveld, AD; Nijkamp, FP; Folkerts, G
The European Respiratory Journal null
Characterization of interleukin-1 receptor-associated kinase in normal and endotoxin-tolerant cells.
L Li et al.
The Journal of biological chemistry, 275(30), 23340-23345 (2000-05-16)
Interleukin-1 receptor-associated kinase (IRAK), a signal transducer for interleukin-1, has also been suggested to participate in the Toll-like receptor-mediated innate immune response to bacterial endotoxin lipopolysaccharide (LPS). Using the human promonocytic THP-1 cell line, we demonstrated that the endogenous IRAK
Neutrophil signaling pathways activated by bacterial DNA stimulation.
Maria E Alvarez, Juan I Fuxman Bass, Jorge R Geffner, Paula X Fernandez Calotti et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Rotavirus Non-Structural Protein-1 suppresses virus induced cellular apoptosis to facilitate viral growth by activating the cell-survival pathways during early stages of infection.
Bagchi P, Dutta D, Chattopadhyay S, Mukherjee A, Halder UC, Sarkar S, Kobayashi N et al.
Journal of virology null
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