推荐产品
生物来源
rabbit
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
E. coli
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
Escherichia coli K12 ... Lexa(948544)
一般描述
抗LexA抗体可检测LexA蛋白和与LexA蛋白融合的重组蛋白。该抗体可用于检测构建用于酵母双杂交系统或其他相互作用捕获系统的潜在诱饵蛋白的表达。LexA会增加约22至25 kDa的蛋白质，具体取决于额外氨基酸的数量。
特异性
识别酵母双杂交系统中使用的诱饵融合蛋白的LexA部分。
免疫原
对应于氨基酸残基1-202的LexA蛋白的带有His标签的DNA结合域
应用
用抗LexA抗体，DNA结合区（兔多克隆抗体）检测LexA，该抗体已显示可在IP & WB中起作用。
质量
通过免疫印迹对LexA-pRB转染酵母裂解液进行了常规评估
目标描述
24 kDa
外形
形式：纯化
分析说明
对照
LexA-pRB转染酵母细胞提取物
LexA-pRB转染酵母细胞提取物
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Yasmine Ligerot et al.
PLoS genetics, 13(12), e1007089-e1007089 (2017-12-09)
Strigolactones (SLs) are well known for their role in repressing shoot branching. In pea, increased transcript levels of SL biosynthesis genes are observed in stems of highly branched SL deficient (ramosus1 (rms1) and rms5) and SL response (rms3 and rms4)
Yu-Fan Chen et al.
Molecular and cellular biology, 36(15), 2039-2050 (2016-05-18)
Cohesin associates with distinct sites on chromosomes to mediate sister chromatid cohesion. Single cohesin complexes are thought to bind by encircling both sister chromatids in a topological embrace. Transcriptionally repressed chromosomal domains in the yeast Saccharomyces cerevisiae represent specialized sites
Notch signaling is antagonized by SAO-1, a novel GYF-domain protein that interacts with the E3 ubiquitin ligase SEL-10 in Caenorhabditis elegans.
Hale, VA; Guiney, EL; Goldberg, LY; Haduong, JH; Kwartler, CS; Scangos, KW; Goutte, C
Genetics null
Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.
Green, SR; Johnson, AD
Molecular Biology of the Cell null
The SOS regulatory system of Escherichia coli.
Little, J W and Mount, D W
Cell, 29, 11-22 (1982)
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