biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
E. coli
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
Escherichia coli K12 ... Lexa(948544)
General description
24 kDa
抗LexA抗体可检测LexA蛋白和与LexA蛋白融合的重组蛋白。该抗体可用于检测构建用于酵母双杂交系统或其他相互作用捕获系统的潜在诱饵蛋白的表达。LexA会增加约22至25 kDa的蛋白质，具体取决于额外氨基酸的数量。
Immunogen
对应于氨基酸残基1-202的LexA蛋白的带有His标签的DNA结合域
Application
用抗LexA抗体，DNA结合区（兔多克隆抗体）检测LexA，该抗体已显示可在IP & WB中起作用。
Biochem/physiol Actions
识别酵母双杂交系统中使用的诱饵融合蛋白的LexA部分。
Physical form
形式：纯化
Analysis Note
对照
LexA-pRB转染酵母细胞提取物
LexA-pRB转染酵母细胞提取物
通过免疫印迹对LexA-pRB转染酵母裂解液进行了常规评估
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Direct identification of insulator components by insertional chromatin immunoprecipitation.
Fujita, T; Fujii, H
Testing null
Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.
Hoshino, A; Fujii, H
Journal of Bioscience and Bioengineering null
Vanessa C Fernandes et al.
The Journal of biological chemistry, 294(15), 5980-5992 (2019-02-16)
Genetic testing for BRCA1, a DNA repair protein, can identify carriers of pathogenic variants associated with a substantially increased risk for breast and ovarian cancers. However, an association with increased risk is unclear for a large fraction of BRCA1 variants
Getting more from the two-hybrid system: N-terminal fusions to LexA are efficient and sensitive baits for two-hybrid studies.
Beranger, F, et al.
Nucleic Acids Research, 25, 2035-2036 (1997)
Notch signaling is antagonized by SAO-1, a novel GYF-domain protein that interacts with the E3 ubiquitin ligase SEL-10 in Caenorhabditis elegans.
Hale, VA; Guiney, EL; Goldberg, LY; Haduong, JH; Kwartler, CS; Scangos, KW; Goutte, C
Genetics null
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