06-702
Anti-phospho-Smad1 (Ser463/465) Antibody
Upstate®, from rabbit
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, Xenopus, rat, mouse
Application:
western blot
Technique(s):
western blot: suitable
Citations:
29
Uniprot accession no.:
产品名称
Anti-phospho-Smad1 (Ser463/465) Antibody, Upstate®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, Xenopus, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer463/pSer465)
Quality Level
Gene Information
human ... SMAD1(4086)
Analysis Note
Control
BMP-4-treated HeLa or NIH/3T3 cells
BMP-4-treated HeLa or NIH/3T3 cells
routinely evaluated by immunoblot on RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4
Application
Detect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
Biochem/physiol Actions
Recognizes the dual serine phosphorylated Smad1 (Ser463/465).
General description
65 kDa
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways.
Immunogen
peptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells.
Boswell, BA; Lein, PJ; Musil, LS
Molecular Biology of the Cell null
Interaction between Smad1 and p97/VCP in rat testis and epididymis during the postnatal development.
Sevil Cayli,Fikret Erdemir,Seda Ocakli,Bahadir Ungor,Hakan Kesici,Tamer Yener,Huseyin Aslan
Reproductive Sciences null
BMP signaling dynamics in embryonic orofacial tissue.
Partha Mukhopadhyay,Cynthia L Webb,Dennis R Warner,Robert M Greene,M Michele Pisano
Journal of Cellular Physiology null
Signal transduction. Mad about SMADs.
J Wrana et al.
Nature, 388(6637), 28-29 (1997-07-03)
TGFbeta-mediated activation of Smad1 in B-cell non-Hodgkin's lymphoma and effect on cell proliferation.
Munoz, O; Fend, F; de Beaumont, R; Husson, H; Astier, A; Freedman, AS
Leukemia null
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