Anti-erbB-2/HER-2 Antibody

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

Recognizes c-erbB-2 (185 kDa), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.

A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein.

Anti-erbB-2/HER-2 Antibody is an antibody against erbB-2/HER-2 for use in IH, IP & WB.

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated erbB-2/HER-2 from 600 μg MCF-7 RIPA lysate, and previously in A431, LNCaP, and c-erbB-2 transfected 3T3/NIH RIPA lysate.

Immunohistochemistry:
A previous lot of antibody has been reported by an independent laboratory, to detect erbB-2/HER-2 in frozen and formalin/paraffin sections of rat breast.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Her-2 on 10 μg of HEK293 lysates.

185 kDa

Replaces: 04-1127

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Frozen solution.

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
A431 cells, HEK293, Jurkat, MCF7 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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