生物来源
rabbit
质量水平
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性（根据同源性预测）
all
制造商/商品名称
Upstate®
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... NOS1(4842)
一般描述
硝基酪氨酸已被确定为细胞损伤和炎症以及NO产生的指标。人们认为，测量硝基酪氨酸的浓度将作为细胞内NO引起损伤的标志物。先前的研究表明，在诸如动脉粥样硬化斑块和类风湿性关节炎等炎症性疾病中发现了硝基酪氨酸。
特异性
识别被硝化的蛋白质。
免疫原
硝化KLH
应用
- 免疫细胞化学分析：在固定和渗透的A-431细胞中，用5mM过氧亚硝酸盐处理15分钟，从代表性批次中提取1:20 00稀释。
- 免疫组织化学的应用：代表性批次在人类结肠癌、人类阿尔茨海默氏症大脑和人类糖尿病肾组织切片中检测到硝基酪氨酸。
- 注意：实际最佳工作稀释度必须由最终用户确定，因为样本和实验条件可能会因最终用户而有所不同
研究子类别
神经递质&受体
神经退行性疾病
神经递质&受体
神经退行性疾病
该兔多克隆硝基酪氨酸抗体（目录号06-284）经验证可用于蛋白质印迹、免疫组织化学、免疫细胞化学中硝基酪氨酸的检测。
质量
通过免疫印迹法对硝基酪氨酸免疫印迹对照（目录号12-354）进行了常规评估
目标描述
硝化BSA的预测分子量：85 kDa
外形
免疫亲和纯化
形式：纯化
纯化兔IgG，溶于含0.1 M Tris-甘氨酸、0.15 M NaCl（pH 7.4）、0.05%叠氮化钠和10 mg/mL BSA的缓冲液中
储存及稳定性
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始样品瓶进行离心。
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Vacuolation in cellular organelles within the central nervous system is a common manifestation of oxidative injury. We found that the spongiform vacuolation observed in PVC-211 murine leukemia virus (PVC-MuLV) neurodegeneration was associated with oxidative damage as detected by immunoreactivity for
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Peroxynitrite (ONOO(-)), a toxic product of the free radicals nitric oxide and superoxide, has been implicated in the pathogenesis of CNS inflammatory diseases, including multiple sclerosis and its animal correlate experimental autoimmune encephalomyelitis (EAE). In this study we have assessed
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Anesthesiology, 94(4), 652-660 (2001-05-31)
Sepsis or peritonitis impairs diaphragmatic contractility and endurance capacity. Peroxynitrite, a powerful oxidant formed by superoxide and nitric oxide, has been implicated in the pathogenesis. Propofol scavenges this reactive molecule. The authors conducted the current study to evaluate whether propofol
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(2), 271-278 (2000-02-05)
Angiotensin II (ANG II) is a well-established participant in many cardiovascular disorders, but the mechanisms involved are not clear. Vascular cell experiments suggest that ANG II is a potent stimulator of free radicals such as superoxide anion, an agent known
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Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 11(3), 193-206 (1999-06-03)
Nitric oxide (NO) has been implicated in the pathogenesis of inflammatory bowel disease since increased NO production is observed in this disease. NO can react with superoxide to generate peroxynitrite which causes and/or exacerbates colitis. Peroxynitrite, in turn, nitrates tyrosine
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