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Merck
CN

06-238

Anti-Gβ Antibody

serum, Upstate®

别名:

Anti-HG2C1, Anti-NEDHYDF, Anti-SSS4

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
21
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biological source

rabbit

conjugate

unconjugated

antibody form

serum

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... GNB2(2783)

General description

35/36kDa

Immunogen

Internal sequence of G-protein β subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base

Application

Detect Gβ using this Anti-Gβ Antibody validated for use in IP & WB.

Biochem/physiol Actions

Gβ subunits; recognizes both isoforms

Physical form

PBS, pH 7.2

Analysis Note

Immunoblot Analysis: A 1:500-1:1000 dilution of this antibody detected the Gβ subunits in 20 μg of a cell lysate prepared from rat brain microsomal preparation (Catalog # 12-144).

Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10 - Combustible liquids

wgk

WGK 2


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H I Krieger-Brauer et al.
The Journal of biological chemistry, 272(15), 10135-10143 (1997-04-11)
Human fat cells possess a multireceptor-linked H2O2-generating system that is activated by insulin. Previous studies revealed that manganese was the sole cofactor required for a hormonal regulation of NADPH-dependent H2O2 generation in vitro. In this report it is shown that
M C Galas et al.
The Journal of biological chemistry, 272(5), 2788-2793 (1997-01-31)
The ADP-ribosylation factor (ARF) GTP-binding proteins are believed to function as regulators of vesicular budding and fusion along the secretory pathway. To investigate the role of ARF in regulated exocytosis, we have examined its intracellular distribution in cultured chromaffin cells
Importance of the G protein gamma subunit in activating G protein-coupled inward rectifier K(+) channels.
T Kawano, L Chen, S Y Watanabe, J Yamauchi, Y Kaziro, Y Nakajima, S Nakajima, H Itoh
Febs Letters null
J Yamauchi et al.
The Journal of biological chemistry, 275(11), 7633-7640 (2000-03-14)
Heterotrimeric G proteins stimulate the activities of two stress-activated protein kinases, c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase in mammalian cells. In this study, we examined whether alpha subunits of G(i) family activate JNK using transient expression system
J Yamauchi et al.
The Journal of biological chemistry, 272(12), 7602-7607 (1997-03-21)
G protein beta and gamma subunits (Gbeta and Ggamma) form a complex that is involved in various signaling pathways. We reported that the C-terminal 10 amino acids of Gbeta are required for association with Ggamma (Yamauchi, J., Kaziro, Y., and

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