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Merck
CN

06-1340

抗磷酸化-IKK-epsilon (Ser172) 抗体

from rabbit, purified by affinity chromatography

别名:

Inhibitor of nuclear factor kappa-B kinase subunit epsilon, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, Inducible I kappa-B kinase, IKK-i

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗磷酸化-IKK-epsilon (Ser172) 抗体, from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_001180251

UniProt accession no.

Q14164

shipped in

wet ice

target post-translational modification

phosphorylation (pSer172)

Quality Level

100

Gene Information

human ... IKBKE(9641)

相关类别

Analysis Note

对照
经CD3+CD28处理和未处理的Jurkat细胞裂解液
已通过蛋白质印迹在经CD3+CD28处理和未经处理的Jurkat细胞裂解液中进行了评估。

蛋白质印迹分析：该抗体的1:1,000稀释液在10 µg CD3+CD28处理和未处理的Jurkat细胞裂解液上检测到IKK-ε。

Application

研究子类别
免疫球蛋白 & 免疫学
研究类别
炎症 & 免疫学
该抗磷酸化IKK-ε（Ser172）抗体经过验证可用在WB中检测磷酸化IKK-ε（Ser172）。

Biochem/physiol Actions

该抗体识别在Ser172处磷酸化的IKK-ε。

Disclaimer

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General description

核因子κ-B激酶亚基ε抑制剂（UniProt：Q14164；也称为EC：2.7.11.10，I-κ-B激酶ε，IKK-E，IKK-ε，IkBKE，诱导型Iκ-B激酶，IKK-i）由人IkBKE（也称为IKKE，IKKI，KIAA0151）基因（基因ID：9641）编码。IKK-ε是一种同型二聚丝氨酸/苏氨酸激酶，在脾脏，胸腺，外周血白细胞，胰腺和胎盘中高度表达。其活性由脂多糖和TNF-α诱导。它通过激活I型IFN，NF-κB和STAT信号传导在调节对病毒感染的炎症反应中起重要作用。IKK-ε可以被IKBKb/IKKb自身磷酸化或磷酸化，并且通过与ATP依赖性RNA解旋酶DDX3X的相互作用增强了丝氨酸172的磷酸化。其ATP结合位点位于氨基酸15至23。据报道，IKK-ε可磷酸化NF-κ-B的抑制剂，导致其泛素化和降解。在激活病毒RNA传感器（例如RIG-I样受体）后，它与DDX3X结合并磷酸化干扰素调节因子(IRF)，IRF3和IRF7以及DDX3X。该活性允许IRF3随后的同源二聚化和核易位，导致促炎和抗病毒基因（包括IFNB）的转录激活。为了建立这样的抗病毒状态，IKBKE形成了几种不同的复合物，其组成取决于细胞和细胞刺激物的类型。已经描述了通过选择性剪接产生的IKK-ε的两种亚型。
观测值〜90 kDa

Immunogen

对应于人IKK-ε磷酸化在Ser10处的线性肽。
表位：磷酸化Ser172

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

亲和纯化
纯化的兔多克隆IgG，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 M NaCl、0.05％叠氮化钠和30%甘油的缓冲液中。

Preparation Note

自收到之日起在-20°C可稳定保存1年。

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存储类别

10 - Combustible liquids

wgk

WGK 1

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