Anti-Cathepsin D Antibody

Cathepsin D is synthesized as a 54kDa precursor which is proteolytically processed to an intermediate 48kDa single chain which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types, especially breast cancer. Cathepsin D is expressed in epithelial cells as well as in macrophages. Immunoreactivity of cancer cells and tissue infiltrating macrophages must be recorded separately.

84% sequence homology to mouse and rat.

This antibody recognizes Cathepsin D.

Epitope: Heavy Chain

GST-tagged recombinant protein corresponding to human Cathepsin D at the heavy chain.

Anti-Cathepsin D Antibody is an antibody against Cathepsin D for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cathepsin D in A431 and HeLa cell lystes.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Apoptosis - Additional

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Cathepsin D on 10 µg of A431 cell lysate.

Observed molecular weight at ~58 kDa Protein is glycosylated and may run slightly higher than predicted.
Predicted molecular weight of the active form is ~46 kDa and ~ 54 kDa for the precursor form.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.