推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
rat, human, mouse
种属反应性（根据同源性预测）
orangutan
技术
immunocytochemistry: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TMEM201(199953)
一般描述
Transmembrane protein 201, also known as NET5, is a transmembrane protein of the nuclear envelope that is homologous to spindle-associated membrane protein 1 (SAMP1) in humans. NET5 is thought to be expressed on the inner nuclear membrane during interphase and move to the polar regions of the mitotic spindle during mitosis.
免疫原
KLH-conjugated linear peptide corresponding to human NET5.
应用
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-NET5 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect NET5 also known as transmembrane protein 201.
Western Blot Analysis: 1 µg/mL from a representative lot detected NET5 on 10 µg of rat heart tissue lysate.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory that detected NET5 in U2OS cells expressing various NET-GFP fusions. (Wilkie, G., et al. (2011) Mol. Cell Proteomics).
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory that detected NET5 in U2OS cells expressing various NET-GFP fusions. (Wilkie, G., et al. (2011) Mol. Cell Proteomics).
质量
Evaluated by Western Blot in human heart tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NET5 on 10 µg of human heart tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NET5 on 10 µg of human heart tissue lysate.
目标描述
~72 and/or 43 kDa observed depending on lysate used. Isoform1: 72 kDa and Isoform 2: 43 kDa Uncharacterized band at ~28 kDa may be observed in some lysates.
外形
Affinity purified
Purified rabbit polyclonal containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Human heart tissue lysate
Human heart tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Anne T Bertrand et al.
Cells, 9(4) (2020-04-05)
LMNA encodes for Lamin A/C, type V intermediate filaments that polymerize under the inner nuclear membrane to form the nuclear lamina. A small fraction of Lamin A/C, less polymerized, is also found in the nucleoplasm. Lamin A/C functions include roles
Phu Le Thanh et al.
Neuromuscular disorders : NMD, 27(4), 338-351 (2017-02-19)
Reports of aberrant distribution for some nuclear envelope proteins in cells expressing a few Emery-Dreifuss muscular dystrophy mutations raised the possibility that such protein redistribution could underlie pathology and/or be diagnostic. However, this disorder is linked to 8 different genes
Nikolaj Zuleger et al.
Genome biology, 14(2), R14-R14 (2013-02-19)
Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells
Nadia Korfali et al.
Nucleus (Austin, Tex.), 3(6), 552-564 (2012-09-20)
One hypothesis to explain how mutations in the same nuclear envelope proteins yield pathologies focused in distinct tissues is that as yet unidentified tissue-specific partners mediate the disease pathologies. The nuclear envelope proteome was recently determined from leukocytes and muscle.
Gavin S Wilkie et al.
Molecular & cellular proteomics : MCP, 10(1), M110-M110 (2010-09-30)
Nuclear envelopes from liver and a neuroblastoma cell line have previously been analyzed by proteomics; however, most diseases associated with the nuclear envelope affect muscle. To determine whether muscle has unique nuclear envelope proteins, rat skeletal muscle nuclear envelopes were
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