推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
M110, monoclonal
种属反应性
rat, human, mouse
制造商/商品名称
Upstate®
技术
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... PRKCA(5578)
特异性
Recognizes PKC α, β, γ. Does not cross react with other PKC isoforms.
免疫原
Recombinant protein of human PKCγ amino acids 499-697.
应用
Anti-PKC (α Antibody, β, γ), clone M110 detects level of PKC (α & has been published & validated for use in ELISA, IP, WB & IC.
质量
Routinely evaluated by immunoblot.
目标描述
80-82 kDa
外形
100 μL of protein A purified mouse monoclonal IgG1 in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
Format: Purified
储存及稳定性
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Thomas T Su et al.
Nature immunology, 3(8), 780-786 (2002-07-16)
NF-kappa B signaling is required for the maintenance of normal B lymphocytes, whereas dysregulated NF-kappa B activation contributes to B cell lymphomas. The events that regulate NF-kappa B signaling in B lymphocytes are poorly defined. Here, we demonstrate that PKC-beta
Lilian Kisiswa et al.
Open biology, 7(1) (2017-01-20)
Tumour necrosis factor receptor 1 (TNFR1)-activated TNFα reverse signalling, in which membrane-integrated TNFα functions as a receptor for TNFR1, enhances axon growth from developing sympathetic neurons and plays a crucial role in establishing sympathetic innervation. Here, we have investigated the
Amos M Sakwe et al.
The Journal of biological chemistry, 280(6), 4436-4441 (2004-12-02)
This study examined the mechanism of Ca2+ entry and the role of protein kinase C (PKC) in Ca2+ signaling induced by activation of the calcium sensing receptor (CaR) in HEK293 cells stably expressing the CaR. We demonstrate that influx of
Michael Rosenberg et al.
Molecular biology of the cell, 17(3), 1364-1374 (2006-01-06)
Nonmuscle myosin II is an important component of the cytoskeleton, playing a major role in cell motility and chemotaxis. We have previously demonstrated that, on stimulation with epidermal growth factor (EGF), nonmuscle myosin heavy chain II-B (NMHC-IIB) undergoes a transient
Nobutaka Takahashi et al.
Journal of neurochemistry, 143(6), 660-670 (2017-10-20)
Cerebellar Purkinje cells (PCs) express two members of the classical protein kinase C (cPKC) subfamily, namely, PKCα and PKCγ. Previous studies on PKCγ knockout (KO) mice have revealed a critical role of PKCγ in the pruning of climbing fibers (CFs)
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