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Merck
CN

05-844

抗-PLK1抗体，克隆35-206

clone 35-206, Upstate®, from mouse

别名:

Polo-like kinase 1, Serine/threonine-protein kinase 13, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, polo like kinase, polo like kinase, polo-like kinase (Drosophila), polo-like kinase 1 (Drosophila)

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

主要文件

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生物来源

mouse

质量水平

100
400

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

35-206, monoclonal

种属反应性

rat, mouse, human

制造商/商品名称

Upstate®

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG2b

NCBI登记号

NP_005021

UniProt登记号

P53350

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... PLK1(5347)
mouse ... Plk1(18817)

相关类别

一般描述

64kda
Polo样激酶1（PLK-1）是一种丝氨酸-苏氨酸蛋白激酶，在细胞周期的M期起着多种重要功能，包括调节中心体成熟和纺锤体组装、去除染色体臂（chromosome arm）的粘连蛋白、APC/C 抑制剂的失活、有丝分裂结束和胞质分裂的调节。 表达的PLK-1 蛋白在G2和M 期聚集至最高水平，在有丝分裂以后和整个G1期下降至几乎不可测的水平，然后在S期再次开始聚集。

免疫原

对应于人Polo样激酶1（PLK1）的6His标记全长融合蛋白。也称丝氨酸-苏氨酸蛋白激酶PLK。

应用

免疫沉淀：一个独立实验室报道，一个先前批次的该抗体可用于PLK1的免疫沉淀。

免疫组织/免疫细胞化学： 一个独立实验室报道，1 μg/mL的先前批次的该抗体在多聚甲醛处理、Triton X-100 固定的细胞中显示出PLK1 的阳性免疫染色。
抗-PLK1抗体（克隆35-206）可检测PLK1水平 & 已发布 & 经验证可用于WB、IP、IC、IH。
研究子类别
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能

生化/生理作用

PLK1

外形

形式：纯化
纯化蛋白A
蛋白A纯化小鼠IgG2b，溶于含有0.07 M Tris-甘氨酸（pH 7.4）、0.105 M NaCl、0.035％叠氮化钠和30％甘油的缓冲液中。 储存于 -20°C。

制备说明

自收到之日起可稳定保存1年。

分析说明

对照
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
已通过蛋白质印迹对3T3/A31细胞的RIPA裂解液进行了评估。

蛋白质印迹分析：0.5-2 μg/mL该抗体在3T3/A31细胞的RIPA裂解液中检测到PLK1。

其他说明

浓度：请参考批次特异性浓缩物的检验报告。

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

分析证书(COA)

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The family of polo-like kinases.
Golsteyn, R M, et al.
Progress in Cell Cycle Research, 2, 107-114 (1996)
Neuronal c-Abl activation leads to induction of cell cycle and interferon signaling pathways.
Schlatterer, SD; Suh, HS; Conejero-Goldberg, C; Chen, S; Acker, CM; Lee, SC; Davies, P
Journal of Neuroinflammation null
Guoning Liao et al.
Scientific reports, 8(1), 12635-12635 (2018-08-24)
Polo-like kinase 1 (Plk1) has been implicated in mitosis, cytokinesis, and proliferation. The mechanisms that regulate Plk1 expression remain to be elucidated. It is reported that miR-100 targets Plk1 in certain cancer cells. Here, treatment with miR-100 did not affect
Chieh-Yuan Cheng et al.
Oncotarget, 9(30), 21231-21243 (2018-05-17)
BI2536 has been developed as a potential therapeutic agent for various cancers but not in oral cancer cells. Since BI2536 exhibits mitosis-regulating activity which are the most radiosensitive, we hypothesized that BI2536 might modulate the radiosensitivity of oral cancer cells.
Zhiguo Li et al.
Cancer research, 77(12), 3169-3180 (2017-05-18)
The mitotic kinase Plk1 contributes to the DNA damage response (DDR) by targeting multiple factors downstream of the core responder kinase ATM/ATR. In this study, we show that Polo-like kinase 1 (Plk1) also phosphorylates key factors upstream of ATM/ATR and
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