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生物来源
mouse
质量水平
偶联物
ALEXA FLUOR™ 555
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
3H10, monoclonal
种属反应性
human
技术
immunocytochemistry: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pSer10)
基因信息
human ... H3C1(8350)
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
特异性
Broad species cross-reactivity expected
Clone 3H10 recoginzes histone N-terminal peptide with Ser10 phosphorylation (pSer10) by bead-based peptide specificity assay. Clone 3H10 does not bind unphosphorylated peptides or peptides with only pThr11 or pSer28 modification.
免疫原
Epitope: H3 pSer10.
应用
Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor™ 555 is an antibody against phospho-Histone H3 for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H3 (Ser10) in HepG2 cells.
The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for peptide inhibition assay (PIA), flow cytometry, immunocytochemistry, immunofluorescence, multiplex assay, Western blotting applications.
The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for peptide inhibition assay (PIA), flow cytometry, immunocytochemistry, immunofluorescence, multiplex assay, Western blotting applications.
质量
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
目标描述
~17 kDa observed.
外形
Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
其他说明
Concentration: Please refer to lot specific datasheet.
法律信息
ALEXA FLUOR is a trademark of Life Technologies
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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