产品名称
Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal, clone 58-10C-31, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
58-10C-31, monoclonal
species reactivity
human, rat, mouse
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IRS1(3667)
Application
Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.
Research Category
Metabolism
Metabolism
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Western Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates.
Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.
Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.
Analysis Note
Control
3T3/A31, 3T3/L1, L6, or MCF7 cell lysates
3T3/A31, 3T3/L1, L6, or MCF7 cell lysates
Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates.
Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
Biochem/physiol Actions
Antibody recognizes the C-terminus of IRS1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.
~ 160 kDa
Immunogen
Epitope: C-terminus
KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein A purified
Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Rongya Tao et al.
Nature medicine, 24(7), 1058-1069 (2018-06-06)
Unsuppressed hepatic glucose production (HGP) contributes substantially to glucose intolerance and diabetes, which can be modeled by the genetic inactivation of hepatic insulin receptor substrate 1 (Irs1) and Irs2 (LDKO mice). We previously showed that glucose intolerance in LDKO mice
Kyle D Copps et al.
The Journal of biological chemistry, 291(16), 8602-8617 (2016-02-06)
Constitutive activation of the mammalian target of rapamycin complex 1 and S6 kinase (mTORC1→ S6K) attenuates insulin-stimulated Akt activity in certain tumors in part through "feedback" phosphorylation of the upstream insulin receptor substrate 1 (IRS1). However, the significance of this
Hua Yan et al.
Molecular medicine reports, 15(1), 180-186 (2016-12-03)
Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease, the pathological process of which is complex. Activation of the c‑Jun N‑terminal kinase (JNK) signaling pathway is associated with the mechanism underlying obesity-induced insulin resistance. Furthermore, the JNK signaling
Himani Kukreti et al.
Journal of cellular biochemistry, 121(5-6), 3070-3089 (2020-02-15)
Aging skeletal muscle shows perturbations in metabolic functions. MicroRNAs have been shown to play a critical role in aging and metabolic functions of skeletal muscle. MicroRNA-34a (miR-34a) is implicated in the brain and cardiac aging, however, its role in aging
Pei-Wen Cheng et al.
Diabetes, 64(10), 3413-3424 (2015-04-18)
Aberrant Wnt signaling appears to play an important role in the onset of diabetes. Moreover, the insulin signaling pathway is defective in the nucleus tractus solitarii (NTS) of spontaneously hypertensive rats (SHRs) and fructose-fed rats. Nevertheless, the relationships between Wnt
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