biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1H6, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable, immunohistochemistry: suitable
isotype
IgG
shipped in
dry ice
target post-translational modification
phosphorylation (pSer)
Quality Level
Gene Information
human ... PTDSS1(9791)
General description
意义:
抗磷脂酰丝氨酸可用于检测膜磷脂磷脂酰丝氨酸(PS)从内膜小叶向外膜小叶的移位;它提供了膜联蛋白V的替代方法,用于定量细胞凋亡。
抗磷脂酰丝氨酸可用于检测膜磷脂磷脂酰丝氨酸(PS)从内膜小叶向外膜小叶的移位;它提供了膜联蛋白V的替代方法,用于定量细胞凋亡。
未给出
磷脂酰丝氨酸(PS)是在所有动植物的膜中发现的磷脂(1,2-二酰基-sn-甘油-(3)-L-磷酸丝氨酸)。 PS最集中在大脑中,因为PS约占神经元脂质含量的10%。神经性PS被认为在维持神经细胞完整性、增强神经递质信号效率、改善神经细胞信号传递方面发挥作用。 细胞凋亡的关键特征之一是细胞外膜上PS的表达。 PS通常在质膜的内侧发现,但是在细胞凋亡的早期阶段,PS易位到外膜。FITC标记的膜联蛋白V可以与暴露的PS结合,为大多数基于膜联蛋白V的细胞凋亡测定提供了基础。
Immunogen
含有70%磷脂酰丝氨酸和30%磷脂酰甘油的脂质体
Application
抗磷脂酰丝氨酸抗体,克隆1H6是用于FC,IH的抗磷脂酰丝氨酸抗体。
流式细胞术:0.2-2 μg/mL先前批次在用Fas配体囊泡处理的Jurkat细胞上检测到细胞表面磷脂酰丝氨酸(目录号01-210)。见第2页。
免疫组织化学:该抗体已由独立实验室报告用于检测用乙醇/丙酮固定的大鼠颈动脉石蜡切片中的磷脂酰丝氨酸(Mandinov,L.,2003)。
免疫组织化学:该抗体已由独立实验室报告用于检测用乙醇/丙酮固定的大鼠颈动脉石蜡切片中的磷脂酰丝氨酸(Mandinov,L.,2003)。
研究子类别
凋亡-附加
凋亡-附加
研究类别
细胞凋亡 & 癌症
细胞凋亡 & 癌症
Biochem/physiol Actions
识别细胞膜中的磷脂酰丝氨酸(PS)
Physical form
形式:纯化
纯化IgG,溶于0.1M Tris-甘氨酸、0.15M NaCl、0.05%叠氮化钠(加30%甘油前)溶液(pH 7.4)。在20°C为液体形式。
蛋白G纯化
Preparation Note
自收到之日起可稳定保存1年。
Analysis Note
对照
脑组织
脑组织
通过流式细胞术对经Fas配体囊泡处理的Jurkat细胞进行常规评估(目录号01-210)
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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wgk
WGK 1
存储类别
10 - Combustible liquids
Manjarika De et al.
Molecular therapy. Nucleic acids, 10, 9-27 (2018-03-04)
There is a pressing need for a ubiquitously expressed antigen or receptor on the tumor surface for successful mitigation of the deleterious side effects of chemotherapy. Phosphatidylserine (PS), normally constrained to the intracellular surface, is exposed on the external surface
Jiunn-Min Shieh et al.
International journal of medical sciences, 10(8), 988-994 (2013-06-27)
Previously, we identified a sequence variant (N375S) of c-Met gene, however, its association with lung cancer risk and prognosis remain undefined. We investigated the genotype distribution of the c-Met-N375S sequence variant in 206 lung cancer patients and 207 non-cancer controls
R S Fernandes et al.
Journal of thrombosis and haemostasis : JTH, 4(7), 1546-1552 (2006-07-15)
That there is a correlation between cancer and procoagulant states is well-known. C6 glioma cell line was originally induced in random-bred Wistar-Furth rats and is morphologically similar to glioblastoma multiforme, the most common aggressive glioma resistant to therapeutic interventions. In
Phosphatidylserine receptor and apoptosis: consequences of a non-ingested meal.
Botto, Marina
Arthritis Research & Therapy, 6, 147-150 (2004)
I Vermes et al.
Journal of immunological methods, 184(1), 39-51 (1995-07-17)
In the early stages of apoptosis changes occur at the cell surface, which until now have remained difficult to recognize. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane
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