Anti-β-Tubulin Antibody, clone AA2

Tubulin β chain (UniProt: Q2KJD0; also known as Tubulin beta-5 chain) is encoded by the TUBB gene (Gene ID: 615087) in human. Tubulin is the major constituent of microtubules (MTs). MTs are cytoskeletal filaments composed ofa/β-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. a/β heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the β-tubulin subunit facing the microtubule plus end conferring a structural polarity. Tubulin binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the a-chain. Tubulin dimers constantly polymerize and depolymerize, and MTs can undergo rapid cycles of assembly and disassembly. The first stage of MT formation, the nucleation phase, is slow. In the presence of Mg2+ and GTP, a and β tubulins join in an end-to-end manner to form protofilaments with alternating a and β subunits. GTP bound to β-tubulin is hydrolyzed to GDP during or immediately after polymerization. This weakens the binding affinity of tubulin for adjacent molecules and favors depolymerization that contributes to the dynamic behavior of MTs. MTs also undergo treadmilling, in which tubulin molecules bound to GDP are continually lost from the minus end and are replaced by the addition of GTP-bound tubulin molecules to the plus end of the same MT. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins and motor proteins. β-Tubulin is reported to undergo phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Clone AA2 is a mouse monoclonal antibody that detects β-Tubulin. It targets an epitope within 19 amino acids from the C-terminal region.

Purified β-tubulin from bovine brain.

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected β-Tubulin in A431 cell lysates.

Tested Applications

Immunofluorescence Analysis: A representative lot detected β-Tubulin in Immunofluorescence applications (Abdelfattah, N., et al. (2018). Nat Commun. 9(1):4541; Kelly, J.J., et al. (2015). J Cell Sci. 128(21):3947-60; Moreira, B.P., et al. (2017). J Cell Sci. 130(6):1179-1193; Verbist, K.C., et al. (2016). Nature. 532(7599):389-93).

Immunocytochemistry Analysis: A representative lot detected β-Tubulin in Immunocytochemistry applications (Verbist, K.C., et al. (2016). Nature. 532(7599):389-93).

Western Blotting Analysis: A representative lot detected β-Tubulin in Western Blotting applications (Abdelfattah, N., et al. (2018). Nat Commun.;9(1):4541; Cho, J-H., et al. (2017). Nat Commun. 8(1):1848; Moreira, B.P., et al. (2017). J Cell Sci. 130(6):1179-1193; Ohshiro, K., et al. (2020). Genes Cancer. 11(1-2):43-52; Rawlinson, S.M., et al. (2018). Nat Commun. 9(1):3057; Shrestha, P., et al. (2020). Nat Neurosci. 23(2):281-292; Terzaghi, L., et al. (2018). Reproduction. 155(3):273-282; Villar-Fincheira, P., et al. (2021). Front Physiol.12:722528).

Immunohistochemistry Applications: A representative lot detected β-Tubulin in Immunohistochemistry applications (Cho, J-H., et al. (2017). Nat Commun. 8(1):1848).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-β-Tubulin, clone AA2, Cat. No. 05-661-I, is a mouse monoclonal antibody that detects β-Tubulin and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.