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Merck
CN

05-637-AF647

Anti-Bmi-1 Antibody, clone F6, Alexa Fluor™ 647

clone F6, from mouse, ALEXA FLUOR™ 647

别名:

Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, Bmi-1

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

Anti-Bmi-1 Antibody, clone F6, Alexa Fluor™ 647, clone F6, from mouse, ALEXA FLUOR™ 647

biological source

mouse

conjugate

ALEXA FLUOR™ 647

antibody form

purified antibody

antibody product type

primary antibodies

clone

F6, monoclonal

species reactivity

human, monkey

species reactivity (predicted by homology)

rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_031578.2

UniProt accession no.

P25916

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... BMI1(648)

Analysis Note

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HeLa cells.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HepG2 cells.
The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor™ 647 is validated for use in Immunocytochemistry for the detection of Bmi-1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

40-44 kDa observed
Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.

Immunogen

Epitope: a.a. 1-202
Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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相关内容

White Paper: Further considerations of antibody validation and usage.
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

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