产品名称
抗磷酸化组蛋白H2A.X(Ser139)抗体,克隆JBW301, clone JBW301, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
JBW301, monoclonal
species reactivity
human, rat, mouse
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer139)
Quality Level
Gene Information
human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)
Analysis Note
蛋白质印迹分析:0.5 µg/mL该抗体可检测到磷酸化组蛋白。星形孢菌素处理的HeLa细胞中200 µg中的X(Ser139)。
Application
先前批次已被证明可用于免疫荧光和染色质免疫沉淀:参见参考文献(Meier,Andreas et al.,2007)
蛋白质印迹分析:0.05-1 μg/mL的该批次在5 μM星形孢菌素(目录号19-123)处理过的Jurkat细胞中酸提取的组蛋白裂解液中检测到磷酸化组蛋白H2A.X(Ser139)。
免疫细胞化学:2 μg/mL先前批次的抗体在用0.5 μM星形孢菌素处理4-6小时的HeLa细胞中检测到磷酸化组蛋白H2A.X。
组蛋白
表观遗传学&核功能
Disclaimer
General description
H2A.X磷酸化是对DNA损伤的非常快速的反应,发生在暴露于电离辐射后的短短一分钟内。H2A.X的磷酸化与DNA DSB的原因无关,并且已观察到磷酸化H2A.X响应导致DSB的环境压力以及程序性细胞事件(包括DNA重排和凋亡)。
Immunogen
Other Notes
Physical form
Preparation Note
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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