05-636-AF647
抗磷酸化组蛋白H2A.X (Ser139) 抗体，克隆JBW301，Alexa Fluor™ 647
clone JBW301, 0.5 mg/mL, from mouse
别名:
Histone H2AX, H2a/x, Histone H2A.X
产品名称
抗磷酸化组蛋白H2A.X (Ser139) 抗体，克隆JBW301，Alexa Fluor™ 647, clone JBW301, 0.5 mg/mL, from mouse
biological source
mouse
conjugate
ALEXA FLUOR™ 647
antibody form
purified antibody
antibody product type
primary antibodies
clone
JBW301, monoclonal
species reactivity
human
species reactivity (predicted by homology)
vertebrates (based on 100% sequence homology)
concentration
0.5 mg/mL
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer139)
Quality Level
Gene Information
human ... H2AX(3014)
Analysis Note
通过免疫细胞化学在未处理和星形孢菌素处理的HeLa细胞中进行评估。
免疫细胞化学分析：该抗体的1:100稀释液可在星形孢菌素处理的HeLa细胞中检测到磷酸化组蛋白H2A.X（Ser139）。
Alexa Fluor™是Life Technologies的注册商标。
免疫细胞化学分析：该抗体的1:100稀释液可在星形孢菌素处理的HeLa细胞中检测到磷酸化组蛋白H2A.X（Ser139）。
Alexa Fluor™是Life Technologies的注册商标。
Application
研究子类别
组蛋白
组蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
该抗磷酸化组蛋白H2A.X（Ser139）抗体（克隆JBW301，Alexa Fluor™ 647）经验证可用于ICC检测磷酸化组蛋白H2A.X（Ser139）。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
作为组蛋白H2A家族的成员，组蛋白H2A.x（H2A.x）是在核小体子集中替代常规H2A的变体组蛋白H2A。H2A.x参与核DNA上双链断裂（DSB）损伤的DNA修复。双链DNA断裂后，H2A.x在丝氨酸139处被ATM激酶迅速磷酸化，并变成γ-H2AFX。该磷酸化步骤可以从DSB的实际位点延伸至数千个核小体，并且可以标记周围的染色质，以募集DNA损伤信号传导和修复所需的蛋白质。作为严重DNA损伤引起的细胞凋亡过程中翻译后修饰的一部分，磷酸化H2A.x的高表达被认为是细胞凋亡的准确指标。
观测值〜17 kDa。关于观察到的分子量信息，参见目录编号05-636。
Immunogen
对应于磷酸化组蛋白H2A.X (Ser139)的线性肽。
Physical form
纯化的小鼠偶联物，溶于含有PBS、15 mg BSA和0.1%叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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