05-632
Anti-14-3-3σ Antibody, clone CS112-2A8
clone CS112-2A8, Upstate®, from mouse
别名:
Stratifin
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
CS112-2A8, monoclonal
Application:
IP, WB
Species reactivity:
mouse, human, rat
Citations:
21
Technique(s):
immunoprecipitation (IP): suitable, western blot: suitable
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CS112-2A8, monoclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SFN(2810)
mouse ... Sfn(55948)
rat ... Sfn(313017)
Analysis Note
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells
Application
Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
30 kDa
Immunogen
GST fusion protein corresponding to full-length human 14-3-3 sigma
Physical form
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Claudio A Pozo et al.
Journal of dairy science, 105(6), 4987-5003 (2022-04-23)
Our aim was to evaluate whether increasing soluble carbohydrates in the herbage by changing the time of the grazing session or including Acacia mearnsii tannin in the diet would affect intake, digestion, N partitioning, and productive performance of dairy cows
A Santana et al.
Journal of dairy science, 106(9), 6060-6079 (2023-07-21)
The aim of this study was to evaluate the inclusion of alfalfa grazing during 8 h continuous or partitioned in 2 separated sessions of 4 h after each milking, on nutrient intake, digestibility, ruminal fermentation, feeding behavior, milk production, milk
Maria A Gallardo et al.
Animal science journal = Nihon chikusan Gakkaiho, 85(11), 935-941 (2014-06-26)
There is no information about the effect of different pasture types on tissue fatty acid profiles of a native rustic lamb breed of the Chiloe Archipelago, the Chilota. Eight Chilota lambs were grazed on a 'Calafatal' pasture (CP), a typical
C Fernández et al.
Journal of dairy science, 104(7), 7830-7844 (2021-04-19)
The aim of this study was to evaluate the effects of incorporating rice straw and orange leaves into the diets for goats. Ten Murciano-Granadina goats at mid lactation weighing 45 ± 0.3 kg were used in a crossover design. Two
Maria D Ioannidou et al.
Foods (Basel, Switzerland), 11(14) (2022-07-28)
The production of cheese can be made from either pasteurized or non-pasteurized milk, depending on the country or dietary habits. In this work, the effect of pasteurization of milk on the progress of the physicochemical properties, fatty acids profile and
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