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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
CS112-2A8, monoclonal
种属反应性
mouse, human, rat
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... SFN(2810)
mouse ... Sfn(55948)
rat ... Sfn(313017)
特异性
Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.
免疫原
GST fusion protein corresponding to full-length human 14-3-3 sigma
应用
Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
质量
routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells
目标描述
30 kDa
外形
Protein G Purified
Format: Purified
Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
储存及稳定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Jonas de Souza et al.
Journal of dairy science, 100(2), 1072-1085 (2016-12-13)
The objective of our study was to investigate the effects of sources of calcium salts of fatty acids (FA) on production, nutrient digestibility, energy balance, and carryover effects of early lactation grazing dairy cows. Treatment diets were offered from 3
J Zha et al.
The Journal of biological chemistry, 272(39), 24101-24104 (1997-09-26)
BAD interacts with anti-apoptotic molecules BCL-2 and BCL-XL and promotes apoptosis. BAD is phosphorylated on serine residues in response to a survival factor, interleukin-3. Phosphorylated BAD cannot bind to BCL-XL or BCL-2 at membrane sites and is found in the
Welder Baldassini et al.
Foods (Basel, Switzerland), 11(20) (2023-07-11)
Wet distiller grains (WDG) are a corn by-product rich in protein and fiber that can be used in feedlot diets. This study evaluated F1 Angus-Nellore bulls fed on a control diet vs. WDG (n = 25/treatment). After a period of
Claudio A Pozo et al.
Journal of dairy science, 105(6), 4987-5003 (2022-04-23)
Our aim was to evaluate whether increasing soluble carbohydrates in the herbage by changing the time of the grazing session or including Acacia mearnsii tannin in the diet would affect intake, digestion, N partitioning, and productive performance of dairy cows
A Santana et al.
Journal of dairy science, 106(9), 6060-6079 (2023-07-21)
The aim of this study was to evaluate the inclusion of alfalfa grazing during 8 h continuous or partitioned in 2 separated sessions of 4 h after each milking, on nutrient intake, digestibility, ruminal fermentation, feeding behavior, milk production, milk
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