推荐产品
生物来源
mouse
质量水平
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
2G9, monoclonal
种属反应性
rat, bovine, mouse, Xenopus, rabbit, pig, human
制造商/商品名称
Upstate®
技术
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
基因信息
human ... PPP2CA(5515)
特异性
PP2A B亚基，亚型反应性是 α > δ,β > γ
免疫原
对应于人PP2A, B亚基的残基398-411的肽。
应用
使用该抗PP2A抗体，B亚基，克隆2G9经验证可用于IH，IP & IB，检测PP2A。
研究子类别
激酶&磷酸酶
神经退行性疾病
激酶&磷酸酶
神经退行性疾病
研究类别
信号传导
神经科学
信号传导
神经科学
质量
已通过免疫印迹对未刺激的A431细胞或小鼠3T3/A31细胞的RIPA裂解液进行了常规评估
目标描述
55kDa
外形
0.1M Tris-甘氨酸（pH 7.4），0.15M NaCl，0.05％叠氮化钠，然后添加甘油至30％
腹水
储存及稳定性
在-20°C下可保存2年
分析说明
对照
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。 加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以 减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。 加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以 减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 1
Jocelyn A Lee et al.
The Journal of biological chemistry, 293(25), 9636-9650 (2018-05-08)
Leucine carboxyl methyltransferase-1 (LCMT-1) methylates the C-terminal leucine α-carboxyl group of the catalytic subunits of the protein phosphatase 2A (PP2A) subfamily of protein phosphatases, PP2Ac, PP4c, and PP6c. LCMT-1 differentially regulates the formation and function of a subset of the
Protein phosphatase 2A regulates estrogen receptor alpha (ER) expression through modulation of ER mRNA stability.
Keen, JC; Zhou, Q; Park, BH; Pettit, C; Mack, KM; Blair, B; Brenner, K; Davidson, NE
The Journal of Biological Chemistry null
Chian Ju Jong et al.
The Journal of biological chemistry, 295(17), 5654-5668 (2020-03-12)
Protein phosphatase 2A (PP2A) is a large enzyme family responsible for most cellular Ser/Thr dephosphorylation events. PP2A substrate specificity, localization, and regulation by second messengers rely on more than a dozen regulatory subunits (including B/R2, B'/R5, and B″/R3), which form
Ilker K Sariyer et al.
Virology, 375(2), 464-479 (2008-03-21)
Previous studies have demonstrated that the JC virus (JCV) late regulatory protein agnoprotein is phosphorylated by the serine/threonine-specific protein kinase-C (PKC) and mutants of this protein at the PKC phosphorylation sites exhibit defects in the viral replication cycle. We have
Jennifer B Jackson et al.
Neoplasia (New York, N.Y.), 14(7), 585-599 (2012-08-21)
Heterotrimeric protein phosphatase 2A (PP2A) consists of catalytic C (PP2Ac), structural A, and regulatory B-type subunits, and its dysfunction has been linked to cancer. Reversible methylation of PP2Ac by leucine carboxyl methyltransferase 1 (LCMT-1) and protein phosphatase methylesterase 1 (PME-1)
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