05-537
抗 FAK 抗体，克隆 4.47
clone 4.47, Upstate®, from mouse
别名:
Anti-FADK, Anti-FADK 1, Anti-FAK, Anti-FAK1, Anti-FRNK, Anti-PPP1R71, Anti-p125FAK, Anti-pp125FAK
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4.47, monoclonal
Application:
ICC, IHC, IP, WB
Citations:
173
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4.47, monoclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PTK2(5747)
General description
125 kDa
粘着斑激酶（FAK）是一种胞质蛋白酪氨酸激酶，已被认为在整联蛋白介导的信号转导途径中起重要作用。FAK在整联蛋白介导的细胞粘附中被激活并酪氨酸磷酸化，并与整联蛋白和其他细胞骨架蛋白在多种粘附细胞的焦点接触中共定位。已经提出FAK与整联蛋白胞质结构域以及细胞骨架蛋白塔林，桩蛋白和/或张力蛋白的物理相互作用通过促进其寡聚化和转磷酸化而在FAK活化中起关键作用。当FAK受到多种信号分子（包括调节胚胎发育，细胞增殖，迁移和凋亡的信号分子）刺激时，酪氨酸磷酸化迅速增加，并且迫切需要有效的研究方法。
Immunogen
GST融合蛋白，对应人FAK（粘着斑激酶）的1-423位残基；对于p125FAK具有特异性
Application
抗FAK抗体，克隆4.47，是一种抗FAK抗体，可用于IC、IH、IP和&WB。
研究子类别
细胞骨架信号转导
细胞骨架信号转导
研究类别
信号传导
信号传导
Biochem/physiol Actions
识别p125FAK；不与Pyk-2发生交叉反应。
Physical form
在免疫亲和纯化免疫球蛋白中蛋白G纯化的免疫球蛋白，溶于0.2M Tris-甘氨酸（pH 7.4）、0.15M NaCl、0.1mM EDTA、0.05％叠氮化钠中，然后再加入甘油至30%。
形式：纯化
蛋白G纯化
Preparation Note
自运送之日起在-20°C可稳定保存2年。避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始样品瓶进行离心。
Analysis Note
对照
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
通过免疫印迹法对3T3/A31 RIPA细胞裂解液，PC-12，Hep-G2，SW 620或Jurkat RIPA细胞裂解液进行常规评估
Other Notes
替代：04-591
浓度：请参考批次特异性浓缩物的分析证书。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
FAK/PYK2 promotes the Wnt/?-catenin pathway and intestinal tumorigenesis by phosphorylating GSK3?.
Gao, C; Chen, G; Kuan, SF; Zhang, DH; Schlaepfer, DD; Hu, J
eLife null
Targeted inhibition of FAK, PYK2 and BCL-XL synergistically enhances apoptosis in ovarian clear cell carcinoma cell lines.
Yoon, H; Choi, YL; Song, JY; Do, I; Kang, SY; Ko, YH; Song, S; Kim, BG
Testing null
Y Shen et al.
Molecular biology of the cell, 10(8), 2507-2518 (1999-08-06)
The focal adhesion kinase (FAK) is discretely localized to focal adhesions via its C-terminal focal adhesion-targeting (FAT) sequence. FAK is regulated by integrin-dependent cell adhesion and can regulate tyrosine phosphorylation of downstream substrates, like paxillin. By the use of a
B van de Water et al.
The Journal of biological chemistry, 274(19), 13328-13337 (1999-05-01)
The relationship between focal adhesion protein (FAK) activity and loss of cell-matrix contact during apoptosis is not entirely clear nor has the role of FAK in chemically induced apoptosis been studied. We investigated the status of FAK phosphorylation and cleavage
Nicoletta I Petridou et al.
PloS one, 7(8), e42577-e42577 (2012-08-11)
The Focal Adhesion Kinase is a well studied tyrosine kinase involved in a wide number of cellular processes including cell adhesion and migration. It has also been shown to play important roles during embryonic development and targeted disruption of the
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