推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
8-5-2, monoclonal
种属反应性
mouse, sheep, human, rat, bovine
制造商/商品名称
Upstate®
技术
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pTyr694/pTyr699)
基因信息
human ... STAT5A(6776)
一般描述
STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.
特异性
Specific for the phosphorylated tyrosine residue 694 of STAT5A (94kDa) and 699 of STAT5B (92kDa). Unidentified proteins may be detected in some lysates.
免疫原
Epitope: Tyr694/699
KLH-conjugated, synthetic phosphopeptide (KAVDG[pY]VKPQIK) corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B. Clone 8-5-2.
应用
Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 8-5-2 is a mouse monoclonal antibody for detection of phospho-STAT5A/B (Tyr694/699) also known as signal transducer & activator of transcription 5A/B & has been validated in WB.
质量
Routinely evaluated by Western Blot on 3T3/A31 cells stimulated with PDGF or HeLa cells stimulated with interferon-α.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected phosphorylated STAT5A/B in lysates from 3T3/NIH cells stimulated with PDGF. 0.5-2 μg/mL of a previous lot detected phosphorylated STAT5A/B in lysates from 3T3/A31 cells stimulated with PDGF and from HeLa cells stimulated with interferon-a (IFN-a).
Western Blot Analysis: 0.5-2 μg/mL of this lot detected phosphorylated STAT5A/B in lysates from 3T3/NIH cells stimulated with PDGF. 0.5-2 μg/mL of a previous lot detected phosphorylated STAT5A/B in lysates from 3T3/A31 cells stimulated with PDGF and from HeLa cells stimulated with interferon-a (IFN-a).
目标描述
STAT5A (94 kDa) and STAT5B (92 kDa)
外形
Format: Purified
Purified mouse monoclonal IgG in buffer containing PBS. Frozen solution at -20°C.
分析说明
Control
IFN-α induced HeLa cells or GM-CSF treated TF-1 cells
IFN-α induced HeLa cells or GM-CSF treated TF-1 cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
A mutant Stat5b with weaker DNA binding affinity defines a key defective pathway in nonobese diabetic mice.
Davoodi-Semiromi, A; Laloraya, M; Kumar, GP; Purohit, S; Jha, RK; She, JX
The Journal of Biological Chemistry null
Carolina S Martinez et al.
Journal of molecular endocrinology, 54(2), 171-184 (2015-02-19)
GH/STAT5 signaling is desensitized in the liver in adult transgenic mice overexpressing GH; however, these animals present greater body size. To assess whether the STAT5 pathway is active during the growth period in the liver in these animals, and how
Forced involution of the functionally differentiated mammary gland by overexpression of the pro-apoptotic protein bax.
Edmund B Rucker,Amber N Hale,David C Durtschi,Kazuhito Sakamoto,Kay-Uwe Wagner
Genesis (2000)
Up-regulation of SLAP in FLI-1-transformed erythroblasts interferes with EpoR signaling.
Lebigot, I; Gardellin, P; Lefebvre, L; Beug, H; Ghysdael, J; Quang, CT
Blood null
T M Schnöder et al.
Cell death and differentiation, 22(6), 974-985 (2014-11-15)
Erythropoiesis is a tightly regulated process. Development of red blood cells occurs through differentiation of hematopoietic stem cells (HSCs) into more committed progenitors and finally into erythrocytes. Binding of erythropoietin (Epo) to its receptor (EpoR) is required for erythropoiesis as
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