推荐产品
生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
20G3, monoclonal
种属反应性
human, mouse
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (non-phospho-Tyr1173)
基因信息
human ... EGFR(1956)
mouse ... Egfr(13649)
特异性
The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.
免疫原
peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor
应用
Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
质量
routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells
目标描述
175kDa
联系
Replaces: 04-337; 04-341
外形
Thiophilic adsoption and size exclusion chromatography
100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
储存及稳定性
2 years at -20°C
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
Alteration of epidermal growth factor receptor activity by mutation of its primary carboxyl-terminal site of tyrosine self-phosphorylation.
The Journal of Biological Chemistry, 263, 3610-3617 (1988)
Multiple autophosphorylation sites of the epidermal growth factor receptor are essential for receptor kinase activity and internalization. Contrasting significance of tyrosine 992 in the native and truncated receptors.
The Journal of Biological Chemistry, 267, 8672-8678 (1992)
Biological procedures online, 13, 1-1 (2011-03-04)
Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was estimated by RT-qPCR
Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice.
Biology Open null
BMC cancer, 16(1), 683-683 (2016-08-27)
The progression of colorectal cancer (CRC) involves recurrent amplifications/mutations in the epidermal growth factor receptor (EGFR) and downstream signal transducers of the Ras pathway, KRAS and BRAF. Whether genetic events predicted to result in increased and constitutive signaling indeed lead
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