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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
5-6, monoclonal
种属反应性
mouse, rat, human
制造商/商品名称
Upstate®
技术
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CRKL(1399)
一般描述
CRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
The antibody does not cross-react with CRKI or CRKII.
The antibody does not cross-react with CRKI or CRKII.
特异性
Other species have not been tested.
the C-terminal SH3 of Human CRKL
免疫原
Epitope: Full-length
Full length GST fusion protein corresponding to Human CRKL
应用
Detect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
质量
Routinely evaluated by Western Blot on K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.
目标描述
39kDa
外形
Protein G Purified
Format: Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
分析说明
Control
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Eike R Hrincius et al.
Cellular microbiology, 12(6), 831-843 (2010-01-22)
The non-structural protein 1 (A/NS1) of influenza A viruses (IAV) harbours several src-homology domain (SH) binding motifs that are required for interaction with cellular proteins. The SH3 binding motif at aa212-217 [PPLPPK] of A/NS1 was shown to be essential for
Cellular interactions of CRKL, and SH2-SH3 adaptor protein
ten Hoeve, J, et al
Cancer Research, 54, 2563-2567 (1994)
Atsushi Oda et al.
The Journal of biological chemistry, 278(8), 6456-6460 (2003-01-11)
Searching for proteins in platelets that can interact with the N-terminal SH3 domain of CrkL (using a combination of a pull-down assay followed by mass spectrometry), we have found that human platelets express an ADP-ribosylation factor (Arf)-specific GTPase-activating protein (GAP)
The proto-oncogene product p120CBL and the adaptor proteins CRKL and c-CRK link c-ABL, p190BCR/ABL and p210BCR/ABL to the phosphatidylinositol-3' kinase pathway
Sattler, M, et al
Oncogene, 12, 839-846 (1996)
IL-4 regulates the expression of CD209 and subsequent uptake of Mycobacterium leprae by Schwann cells in human leprosy.
Teles RM, Krutzik SR, Ochoa MT, Oliveira RB, Sarno EN, Modlin RL
Infection and Immunity null
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