生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
MPM-2, monoclonal
种属反应性
vertebrates
制造商/商品名称
Upstate®
技术
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
运输
wet ice
基因信息
human ... FOXM1(2305)
一般描述
This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
特异性
Recognizes phosphorylated serines or threonines when followed by a proline.
免疫原
Mitotic human HeLa cell cytosolic lysate
应用
Detect phospho-Ser/Thr-Pro MPM-2 also known as Mitotic protein #2 with Anti-phospho-Ser/Thr-Pro MPM-2 Antibody (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
质量
routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
目标描述
varies depending on the protein detected
外形
Protein G Purified
Format: Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in mouse IgG in 1.0 mL PBS containing no preservatives.
储存及稳定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Colcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde
Colcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Rong Sang et al.
Cell & bioscience, 12(1), 78-78 (2022-06-02)
Mental retardation is a complex neurodevelopmental disorder. NPAT, a component of the histone locus body (HLB), has been implicated as a candidate gene for mental retardation, with a mechanism yet to be elucidated. We identified that mxc, the Drosophila ortholog
Phosphoproteins are components of mitotic microtubule organizing centers.
Vandre, D D, et al.
Proceedings of the National Academy of Sciences of the USA, 81, 4439-4443 (1984)
Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism.
Yaffe, M B, et al.
Science (New York, N.Y.), 278, 1957-1960 (1997)
Makoto T Hayashi et al.
Nature, 522(7557), 492-496 (2015-06-26)
Tumour formation is blocked by two barriers: replicative senescence and crisis. Senescence is triggered by short telomeres and is bypassed by disruption of tumour-suppressive pathways. After senescence bypass, cells undergo crisis, during which almost all of the cells in the
Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.
Kuang, J, et al.
Proceedings of the National Academy of Sciences of the USA, 86, 4982-4986 (1989)
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