Anti-RUNX2 Antibody, clone AS110

RUNX2 (Runt-related transcription Factor) is a transcription factor that binds to its canonical consensus sequence 5’- PYGPYGGT-3’ in a number of promoters and enhancers. They include LCK, T-cell receptor enhancers, and various bone related genes such as osteocalcin, osteopontin, and bone sialoprotein. As a result of this, RUNX2 is involved in osteoblastic differentiation and skeletal morphogenesis. RUNX2 contains a proline/serine/ threonine-rich region at its C-terminus that has shown to be critical for its transcriptional activity.

Detects RUNX2

Other species have not been tested.

Epitope: Internal

GST-coupled recombinant protein corresponding to amino acids 311-415 of human RUNX2.

Anti-RUNX2 Antibody, clone AS110 detects level of RUNX2 & has been published & validated for use in WB & IC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Routinely evaluated by Western Blot.

Western Blot Analysis:
Anti-RUNX2, clone AS110 detected RUNX at 1:1,000 to 1:2,000 dilution in HL-60 cell lysate resolved via SDS-PAGE and transferred to PVDF.

54-57 kDa

Protein G Purified

Format: Purified

Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3.

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.