05-1459
Anti-SMAD1 Antibody, clone AS22
clone AS22, from mouse
别名:
SMAD 1, SMAD family member 1, SMAD, mothers against DPP homolog 1, MAD, mothers against decapentaplegic homolog 1, Mad-related protein 1, Mothers against DPP homolog 1, Sma- and Mad-related protein 1, TGF-beta signaling protein 1, Transforming growth fac
产品名称
Anti-SMAD1 Antibody, clone AS22, clone AS22, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
AS22, monoclonal
species reactivity
mouse, human
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SMAD1(4086)
Analysis Note
Routinely evaluated by Western Blot
Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
Application
Anti-SMAD1 Antibody, clone AS22 detects level of SMAD1 & has been published & validated for use in WB & IF.
Research Category
Signaling
Signaling
Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
Biochem/physiol Actions
Detects SMAD1
Other species were not tested.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
52 kDa
Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.
Immunogen
GST-fusion protein corresponding to amino acids 1-110 of human SMAD1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1100
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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