05-1427
Anti-ARF1 Antibody, clone 3F1
clone 3F1, from mouse
别名:
ADP-ribosylation factor 1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F1, monoclonal
Application:
ELISA
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
rat, human, mouse
Citations:
1
Technique(s):
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
产品名称
Anti-ARF1 Antibody, clone 3F1, clone 3F1, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F1, monoclonal
species reactivity
rat, human, mouse
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ARF1(375)
mouse ... Arf1(11840)
Analysis Note
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
Application
Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
Biochem/physiol Actions
Recognizes human ARF1
General description
ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
~21 kDa
Immunogen
Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
Other Notes
Replaces: MAB3779
Physical form
Format: Purified
Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Erica Tagliatti et al.
eLife, 5 (2016-01-06)
Recycling of synaptic vesicles (SVs) is a fundamental step in the process of neurotransmission. Endocytosed SV can travel directly into the recycling pool or recycle through endosomes but little is known about the molecular actors regulating the switch between these
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