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生物来源
rabbit
质量水平
抗体形式
culture supernatant
抗体产品类型
primary antibodies
克隆
A11W, monoclonal
种属反应性
rat, chimpanzee, human, mouse, bovine
技术
western blot: suitable
同位素/亚型
IgG
适用性
not suitable for immunoprecipitation
UniProt登记号
运输
dry ice
靶向翻译后修饰
phosphorylation (pTyr694/pTyr699)
基因信息
bovine ... Stat5A(282375)
一般描述
The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.
特异性
Phospho-STAT5A/B (Tyr694/699).
免疫原
Epitope: pTyr694/699
KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B.
应用
Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB.
Research Category
Signaling
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Research Sub Category
Transcription Factors
Transcription Factors
质量
Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.
目标描述
~95 kDa
联系
Replaces: 05-886
外形
Cultured supernatant containing 0.05% sodium azide.
储存及稳定性
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
PDGF treated 3T3/NIH cells
PDGF treated 3T3/NIH cells
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Blood, 97(1), 221-226 (2001-01-03)
JAB/suppressor of cytokine signaling 1 (SOCS1) STAT-induced STAT inhibitor-1 (SSI-1) (JAB/SOCS1/SSI-1) is an SH2-domain-containing protein that is induced by and negatively regulates signaling by a number of cytokines including interleukin-4 (IL-4), IL-6, interferon (IFN)-gamma, prolactin, growth hormone, and erythropoietin. The
Altered interleukin-12 responsiveness in Th1 and Th2 cells is associated with the differential activation of STAT5 and STAT1.
Blood, 91, 1341-1354 (1998)
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