04-841
Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal
culture supernatant, clone A9W4, from rabbit
别名:
RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
A9W4, monoclonal
Application:
immunoprecipitation (IP)
western blot
western blot
Species reactivity:
rat, human, mouse, bovine
Citations:
6
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal, culture supernatant, clone A9W4, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
A9W4, monoclonal
species reactivity
rat, human, mouse, bovine
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
rat ... Rock2(25537)
Analysis Note
Control
RIPA lysates from PC-12 cells
RIPA lysates from PC-12 cells
Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.
Application
Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.
Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Predicted to cross-react with mouse and bovine based on sequence homology.
Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
150 kDa
Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.
Immunogen
6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.
Other Notes
Replaces: 05-841
Physical form
Cultured supernatant containing 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
T Leung et al.
Molecular and cellular biology, 16(10), 5313-5327 (1996-10-01)
The GTPase RhoA has been implicated in various cellular activities, including the formation of stress fibers, motility, and cytokinesis. We recently reported on a p150 serine/threonine kinase (termed ROK alpha) binding RhoA only in its active GTP-bound state and on
S Vincent et al.
Molecular and cellular biology, 17(4), 2247-2256 (1997-04-01)
The Ras-related Rho family GTPases mediate signal transduction pathways that regulate a variety of cellular processes. Like Ras, the Rho proteins (which include Rho, Rac, and CDC42) interact directly with protein kinases, which are likely to serve as downstream effector
Ines Jeric et al.
Nature communications, 7, 13781-13781 (2016-12-22)
Hepatocellular carcinoma (HCC) is a leading cause of cancer deaths, but its molecular heterogeneity hampers the design of targeted therapies. Currently, the only therapeutic option for advanced HCC is Sorafenib, an inhibitor whose targets include RAF. Unexpectedly, RAF1 expression is
Karen A Newell-Litwa et al.
The Journal of cell biology, 210(2), 225-242 (2015-07-15)
RhoGTPases organize the actin cytoskeleton to generate diverse polarities, from front-back polarity in migrating cells to dendritic spine morphology in neurons. For example, RhoA through its effector kinase, RhoA kinase (ROCK), activates myosin II to form actomyosin filament bundles and
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