Anti-phospho-PPARγ (Ser82) Antibody, clone AW504, rabbit monoclonal

Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: ,  and . PPAR stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs can bind to DNA only as a heterodimer with the retinoid X receptor (RXR).

PPARγ phosphorylated at Ser82, Mr 55 & 60 kDa.

Predicted to cross-react with rat based on sequence homology.

Epitope: pSer82

KLH-conjugated, synthetic peptide containing the sequence PApSPP in which pS corresponds to phosphoserine at residue 82 of human PPARγ.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-phospho-PPAR gamma antibody, clone AW504 (rabbit monoclonal antibody) validated in WB to detect phospho-PPARγ (Ser82) also known as Nuclear receptor subfamily 1 group C member 3 & PPAR gamma.

Evaluated by western blot on NIH/3T3 L1 cells that were undifferentiated and differentiated into adipocytes.
Western Blot Analysis: 1:500 dilution of this antibody detected PPARγ phosphorylated at Ser82 in undifferentiated and acipocyte differentiated NIH/3T3 L1 cells.

~55 & 60 kDa

Replaces: 05-816

Cultured supernantant containing 0.05% sodium azide.

Format: Purified

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
3T3/L1 cells differentiated into adipocytes, PMA-stimulated THP-1 cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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