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生物来源
rabbit
质量水平
抗体形式
culture supernatant
抗体产品类型
primary antibodies
克隆
MC83, monoclonal
种属反应性
human
制造商/商品名称
Upstate®
技术
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
phosphorylation (pThr11)
基因信息
human ... H3C1(8350)
一般描述
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. In mammals, phosphorylation of Thr11 modification is restricted to the centromeric region while in plant cells, phosphorylation of Thr11 was distributed along the entire length of condensed chromosomes. Mitosis-specific phosphorylation of histone H3 at Thr11 is mediated by Dlk/ZIP kinase.
特异性
Broad species cross-reactivity is expected based on sequence homology. An additional unidentified band at ~40 kDa appears in some lysates.
Recognizes histone H3 phosphorylated at Thr11, Mr 17kDa.
免疫原
Peptide containing ...KS[pT]G..., in which pT corresponds to phospho-threonine at position 11 of human histone H3
应用
Anti-phospho-Histone H3 (Thr11) Antibody, clone MC83 is a rabbit monoclonal antibody for detection of phospho-Histone H3 (Thr11) also known as H3T11P, Histone H3 (phospho T11) & has been validated in WB, PIA, Mplex.
Immunoblot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant histone H3 (Catalog # 14-494).
Western Blot Analysis:
A 1:5,000 dilution of a previous lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant histone H3 (Catalog # 14-494).
Multiplexing:
Beadlyte Histone-Peptide Specificity Assay: 1:1,000-1:50,000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. Only the peptide containing phospho-threonine 11 was
detected (Figure C).
Peptide Inhibition Analysis: 1 μM of histone H3 peptide containing phospho-threonine 11 abolished detection of histone H3 by anti-phospho-Histone H3 (Thr11), clone MC83 in immunoblot analysis of acid extracts from
colcemid treated HeLa cells (Figure D, lane 4).
A 1:500-1:2000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant histone H3 (Catalog # 14-494).
Western Blot Analysis:
A 1:5,000 dilution of a previous lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid, but not unmodified recombinant histone H3 (Catalog # 14-494).
Multiplexing:
Beadlyte Histone-Peptide Specificity Assay: 1:1,000-1:50,000 dilutions of a previous lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. Only the peptide containing phospho-threonine 11 was
detected (Figure C).
Peptide Inhibition Analysis: 1 μM of histone H3 peptide containing phospho-threonine 11 abolished detection of histone H3 by anti-phospho-Histone H3 (Thr11), clone MC83 in immunoblot analysis of acid extracts from
colcemid treated HeLa cells (Figure D, lane 4).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
质量
Routinely evaluated by immunoblot analysis.
目标描述
17kDa
联系
Replaces: MABE468
外形
Cultured supernantant in 0.05% sodium azide
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
分析说明
Control
HeLa acid extracts
HeLa acid extracts
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Genetics, 207(4), 1313-1333 (2017-10-08)
Saccharomyces cerevisiae Mek1 is a CHK2/Rad53-family kinase that regulates meiotic recombination and progression upon its activation in response to DNA double-strand breaks (DSBs). The full catalog of direct Mek1 phosphorylation targets remains unknown. Here, we show that phosphorylation of histone
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