04-773
Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal
culture supernatant, clone SA19, from rabbit
别名:
Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A
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所有图片(1)
About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
生物来源
rabbit
质量水平
抗体形式
culture supernatant
抗体产品类型
primary antibodies
克隆
SA19, monoclonal
种属反应性
rat, chicken, mouse, human
技术
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
phosphorylation (pThr850)
基因信息
human ... PPP1R12A(4659)
一般描述
Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.
特异性
Predicted to cross-react with mouse and rat.
Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.
免疫原
Epitope: pThr850(Ch) or pThr853(H)
KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
应用
Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.
质量
Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).
目标描述
Doublet at ~130 kDa
联系
Replaces: 05-773
分析说明
Control
HEK293 cell lysate phosphorylated by ROKα/ROCKII.
HEK293 cell lysate phosphorylated by ROKα/ROCKII.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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