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Merck
CN

04-721

抗Dicer1抗体，克隆5D12.2

clone 5D12.2, Upstate®, from mouse

别名:

DCR1, Dicer, HERNA, KIAA0928

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗Dicer1抗体，克隆5D12.2, clone 5D12.2, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

5D12.2, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μL

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_085124.2

UniProt accession no.

Q9UPY3

shipped in

ambient

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... DICER1(23405)

相关类别

Analysis Note

通过蛋白质印迹对 HeLa 核提取物的裂解液进行了常规评估。

Application

抗 Dicer1 抗体，克隆 5D12.2 是一种用于检测 Dicer1（也称为 DCR1、Dicer、HERNA、KIAA0928）的小鼠单克隆抗体，&已在 WB 中得到验证。
研究子类别
RNA 代谢&结合蛋白

RNA 结合蛋白（RBP）
研究类别
表观遗传学&核功能
蛋白质印迹

Biochem/physiol Actions

人 Dicer1

Disclaimer

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General description

220kda
RNA 干扰（RNAi）是转录后基因沉默的机制，导致基因活性丧失。当长的 dsRNA 被 Dicer1（dsRNA 特异性核酸内切酶的 RNase III 家族）酶切割成 21-23 个核苷酸的 siRNA（小干扰 RNA）时，RNAi 被激活。这些 siRNA 与蛋白质结合形成 RISC（RNA 诱导的沉默复合体），当双链 siRNA 解开成单链 siRNA 时，RISC 被激活。激活的 RISC 识别并结合 mRNA 中的互补序列。mRNA 被切割，总体效果是抑制蛋白质表达和基因活性的丧失。Dicer1 与 Argonaute 蛋白质家族（Ago1 和 Ago2）共享相同的结构域（PIWI 和 PAZ 结构域），这些区域为 RNA 诱导的沉默复合物（RISC）提供核酸内切酶活性。

Immunogen

对应于人 Dicer1 氨基酸 378-385 的 KLH 偶联的合成肽

Other Notes

请参考特定批次的数据表。

Physical form

形式：纯化的
蛋白质 G 色谱

Preparation Note

自收到之日起，在 2-8° 以未稀释的等分试样可保存 6 个月。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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相关内容

RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

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