推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
5F10, monoclonal
种属反应性
human
制造商/商品名称
Upstate®
技术
western blot: suitable
同位素/亚型
IgG1
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pThr669)
基因信息
human ... EGFR(1956)
特异性
Recognizes EGFR when phosphorylated at Threonine 669.
免疫原
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
应用
Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
质量
Routinely evaluated by immunoblot.
目标描述
180 kDa
外形
Thiophilic adsoption and size exclusion chromatography
100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Format: Purified
储存及稳定性
6 months at -20°C from date of shipment
分析说明
Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control
Includes PMA and pervanadate treated A431 cell lysate as a positive control
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
10 - Combustible liquids
G J Heisermann et al.
The Journal of biological chemistry, 263(26), 13152-13158 (1988-09-15)
The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which
Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
Heisermann, G J, et al
The Journal of Biological Chemistry, 265, 12820-12827 (1990)
Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
Winograd-Katz, S E and Levitzki, A
Oncogene, 25, 7381-7390 (2006)
F Yan et al.
Oncogene, 36(50), 6919-6928 (2017-09-05)
Overexpression of DNMT1 and KIT is prevalent in lung cancer, yet the underlying molecular mechanisms are poorly understood. While the deregulated activation of DNMT1 or KIT has been implicated in lung cancer pathogenesis, whether and how DNMT1 and KIT orchestrate
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门