04-1488
抗-HIRA抗体，克隆WC119
clone WC119, from mouse
别名:
DiGeorge critical region gene 1, HIR (histone cell cycle regulation defective) homolog A (S. cerevisiae), HIR histone cell cycle regulation defective homolog A, HIR histone cell cycle regulation defective homolog A (S. cerevisiae), TUP1-like enhancer of
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
WC119, monoclonal
Application:
immunoprecipitation (IP)
western blot
western blot
Species reactivity:
mouse
Citations:
13
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-HIRA抗体，克隆WC119, clone WC119, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
WC119, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HIRA(7290)
Analysis Note
对照
NIH/3T3细胞裂解液
NIH/3T3细胞裂解液
通过蛋白质印迹在NIH/3T3细胞裂解液中进行了评估。
蛋白质印迹分析：0.44 µg/mL抗体可在10 µg NIH/3T3细胞裂解液中检测到HIRA。
蛋白质印迹分析：0.44 µg/mL抗体可在10 µg NIH/3T3细胞裂解液中检测到HIRA。
Application
免疫沉淀分析： 先前批次已在一个独立实验室中进行了IP测试。(Hall, C., et al. (2001).Molecular and Cellular Biology.21(5):1854-1865.)
抗HIRA抗体，克隆WC119是用于检测HIRA（也称为HIR（组蛋白细胞周期调节缺陷）同源物）的小鼠单克隆抗体，&已在WB & IP中进行了验证。
研究子类别
染色质生物学
Chaperones
染色质生物学
Chaperones
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
该抗体可识别HIRA。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
HIRA（分裂蛋白1的TUP1样增强子）是一种主要位于细胞核中的蛋白。它充当组蛋白chaparone，优先将组蛋白H3.3置于核小体上。在多种组织中广泛表达，并在胚胎发生过程中表达。 数据表明，HIRA在转录调控机制中起着与酵母HIR1和HIR2共同作用相似的作用。已显示HIRA蛋白与HIRIP3，HIRIP5，组蛋白H2B和H4以及PAX3相互作用。HIRA可能在DiGeorge综合征（DGS）（一种发育障碍）的病因中起作用。该疾病的临床特征包括胸腺和甲状旁腺的缺失或发育不全，心血管畸形，面部发育不良，腭裂和智力低下。
~112 kDa
Immunogen
标记GST的重组蛋白，对应于人HIRA。
表位：未知
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
纯化的小鼠单克隆IgG1κ溶于含0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl和0.05％叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Lorea Valcarcel-Jimenez et al.
Science advances, 8(42), eabq8297-eabq8297 (2022-10-22)
Fumarate hydratase (FH) is a mitochondrial enzyme that catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (TCA) cycle. Germline mutations of FH lead to hereditary leiomyomatosis and renal cell carcinoma (HLRCC), a cancer syndrome characterized by
Jong-Sun Lee et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(23), E3213-E3220 (2016-05-25)
The histone chaperone HIRA complex, consisting of histone cell cycle regulator (HIRA), Ubinuclein1 (UBN1), and calcineurin binding protein 1 (CABIN1), deposits histone variant H3.3 to genic regions and regulates gene expression in various cellular processes, including cellular senescence. How HIRA-mediated
Xiaowei Xu et al.
Nature communications, 13(1), 2514-2514 (2022-05-07)
Newly synthesized H3.1 and H3.3 histones are assembled into nucleosomes by different histone chaperones in replication-coupled and replication-independent pathways, respectively. However, it is not clear how parental H3.3 molecules are transferred following DNA replication, especially when compared to H3.1. Here
Hai-Tong Fang et al.
Nature communications, 9(1), 1537-1537 (2018-04-20)
H3.3 is a histone variant, which is deposited on genebodies and regulatory elements, by Hira, marking active transcription. Moreover, H3.3 is deposited on heterochromatin by Atrx/Daxx complex. The exact role of H3.3 in cell fate transition remains elusive. Here, we
Tetsuro Komatsu et al.
Journal of virology, 86(12), 6701-6711 (2012-04-13)
In infected cells, the chromatin structure of the adenovirus genome DNA plays critical roles in its genome functions. Previously, we reported that in early phases of infection, incoming viral DNA is associated with both viral core protein VII and cellular
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