GFP, FLAG-tagged SNRNP70 mRNA

Both mRNAs and non-coding RNAs interact with RNA-binding proteins and regulate critical cellular processes and their dysregulation contributes to multiple diseases. Thus, analyzing RNA-protein interactions are critical for understanding the mechanism of diseases and designing clinical strategies.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.

The GFP, FLAG-tagged SNRNP70 mRNA was developed to enable to perform control RIP experiments with RIPAb+ FLAG (Cat. No. 03-901) or RIPAb+ GFP (Cat. No. 03-902) RIP validated antibody and primer kits, and Magna RIP kit (Cat. # 17-700).

Store at -80ºC

The high amount of GFP-FLAG-tagged SNRNP70 protein is expressed immediately after transfection to cells and the well-known specific interaction of SNRNP70 and U1snRNA can be used for control RIP experiments with anti-GFP or anti-FLAG antibodies.

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